| 產(chǎn)品編號 | bsm-61107R |
| 英文名稱 | Phospho-PP2A alpha (Ser307) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化蛋白磷酸酶2A重組兔單抗 |
| 別 名 | eplication protein C; PP2A-alpha; Replication protein C; RP-C; PPP2CA; MGC786. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | 35 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PP2A alpha around the phosphorylation site of S307 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. SWISS: P67775 Gene ID: 5516 激酶和磷酸酶（Kinases and Phosphatases） PP-2A 蛋白質(zhì)磷酸脂酶主要用于細胞信號傳導(dǎo)，參與酵母細胞及兩棲類卵母細胞有絲分裂的蛋白絲/蘇氨酸磷酸酶。由65kDa和55kDa（亞基A和B）調(diào)節(jié)亞基及37kDa（亞基C）催化亞基組成。催化亞基在物種間高度保守。此酶的表達與細胞周期調(diào)節(jié)有關(guān)。此抗體與PP-2A subunit A,B 均有交叉反應(yīng)。 |
| 產(chǎn)品圖片 |
Western blot analysis of (1) Rat kidney lysate; (2) A431 cell lysate treated with EGF. Using Phospho-PP2A alpha (Ser307) (bsm-61107R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
