| 產(chǎn)品編號 | bsm-61781R |
| 英文名稱 | Bub1 Recombinant Rabbit mAb |
| 中文名稱 | 紡錘體檢查點(diǎn)蛋白重組兔單抗 |
| 別 名 | Bub1; BUB1A; BUB1L; hBUB1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 122 kDa |
| 檢測分子量 | 122 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BUB1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. SWISS: O43683 Gene ID: 699 紡錘體檢查點(diǎn)（spindle checkpoint)是細(xì)胞周期中的一個重要檢查點(diǎn)，由Mad2、Bub1等染色體動粒(kinetochore)蛋白構(gòu)成，像“傳感器”一樣能感知微管與動粒結(jié)合情況和張力。紡錘體檢查點(diǎn)阻滯細(xì)胞進(jìn)入分裂后期直到所有染色體著絲點(diǎn)正確與微管聯(lián)接,確保染色體均等分配,維持基因組穩(wěn)定性。 |
| 產(chǎn)品圖片 |
Western blot analysis of K562 cell lysate. Using Bub1 (bsm-61781R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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