| 產(chǎn)品編號 | bsm-61819R |
| 英文名稱 | Phospho-GCN2 (Thr899) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化蛋白激酶GCN2蛋白重組兔單抗 |
| 別 名 | GCN2-like protein; GCN2; KIAA1338; EIF2AK4. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:1000-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 187 kDa |
| 檢測分子量 | 220 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GCN2 around the phosphorylation site of T899 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability.Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation. SWISS: Q9P2K8 Gene ID: 440275 GCN2蛋白的作用；可能是將短期記憶變成長期記憶的一個主控調節(jié)因子， GCN2蛋白在長期記憶形成中功能的發(fā)現(xiàn)將可能幫助研究人員開發(fā)出提高記憶力喪失的病人提高記憶力。 |
| 產(chǎn)品圖片 |
Western blot analysis of (1) HeLa cell lysate; (2) HeLa cell lysate treated with Calyculin. Using Phospho-GCN2 (Thr899) (bsm-61819R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
