| 產(chǎn)品編號 | bsm-62173R |
| 英文名稱 | TSC1 Recombinant Rabbit mAb |
| 中文名稱 | 結(jié)節(jié)性硬化癥蛋白1重組兔單抗 |
| 別 名 | Hamartin; kiaa0243; LAM; TSC; Tsc1; Tuberous sclerosis 1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 130 kDa |
| 檢測分子量 | 150 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Hamartin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Non-catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. SWISS: Q92574 Gene ID: 7248 ????結(jié)節(jié)性硬化癥為常染色體顯性遺傳，也常見散發(fā)病例。是腫瘤抑制基因，基因產(chǎn)物分別為Hamartin和tuberin，兩者均調(diào)節(jié)細胞生長。 ????結(jié)節(jié)性硬化癥(tuberous sclerosis)又稱結(jié)節(jié)性腦硬化，Bourneville病。本病可歸類于神經(jīng)皮膚綜合征(亦稱斑痣性錯構(gòu)瘤病)，是源于外胚層的器官發(fā)育異常所致，病變累及神經(jīng)系統(tǒng)、皮膚和眼，也可累及中胚層，內(nèi)胚層器官如心、肺、骨，腎和胃腸等。皮脂腺瘤是皮膚神經(jīng)末梢、增生的結(jié)締組織和血管組成，視網(wǎng)膜可見膠質(zhì)瘤、神經(jīng)節(jié)細胞瘤，心、腎、肺、 |
| 產(chǎn)品圖片 |
Western blot analysis of HeLa cell lysate. Using TSC1 (bsm-62173R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
