| 產(chǎn)品編號 | bs-0094R |
| 英文名稱 | Rabbit Anti-AACT antibody |
| 中文名稱 | α-1抗胰糜蛋白酶抗體 |
| 別 名 | AACT_HUMAN; SERPINA3; alpha-1-antichymotrypsin; ACT; Cell growth-inhibiting gene 24/25 protein; Serpin A3; GIG24; GIG25; serpin family A member 3; |
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Specific References (1) | bs-0094R has been referenced in 1 publications.
[IF=3.13] Wang, Robert YL, et al. "Proteome Demonstration of Alpha-1-Acid Glycoprotein and Alpha-1-Antichymotrypsin." Pediatric Infectious Disease Journal (2014). WB ; Human.
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| 研究領域 | 心血管 細胞生物 神經(jīng)生物學 糖尿病 泛素 Alzheimer's |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AACT: 121-320/423 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Alpha 1-Antichymotrypsin, a member of the serine proteinase inhibitor (serpin) family, inhibits neutrophil proteinase cathepsin G and mast cell chymases, and protects the lower respiratory tract from damage by proteolytic enzymes. It contains a reactive centre loop, which interacts with cognate proteinases, resulting in loop cleavage and a major conformational change. Recently, alpha 1-antichymotrypsin has been identified as a major constituent of the neurofibrillary plaques associated with Alzheimers disease, and in vitro studies have shown that it enhances the rate of amyloid-fibril formation. These observations and recent genetic evidence suggest that alpha 1-antichymotrypsin is important in the pathogenesis of Alzheimers disease. Function: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. Subunit: Interacts with DNAJC1. Subcellular Location: Secreted. Tissue Specificity: Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. Similarity: Belongs to the serpin family. SWISS: P01011 Gene ID: 12 Database links: Entrez Gene: 12 Human Omim: 107280 Human SwissProt: P01011 Human Unigene: 534293 Human Unigene: 710488 Human AACT是由神經(jīng)膠質細胞分泌并參與淀粉樣纖維生成和沉積的一種色氨酸蛋白酶抑制劑,被認為是Aβ肽的假性底物，與Aβ肽具有很高的親合性，可促進Aβ肽的纖維化以生成淀粉樣沉積。 ApoE作為AD的另一種病理伴隨性物質，也可識別并與Aβ肽相結合，促進Aβ肽沉積生成淀粉樣老年斑塊。由于AACT和ApoE在AD發(fā)病中具有共同的作用途徑，因此探討其相互作用可能是AD治療中一個新的途徑。 AACT是一種絲氨酸蛋白酶抑制劑，可以中和糜蛋白酶等酶活性的蛋白酶，存在于大多數(shù)的組織細胞、巨噬細胞以及多種胃腸道和肺部腫瘤中，但多形核白細胞中無此物質，它可以作為組織細胞瘤和良性/惡性纖維組織細胞瘤的標志。此抗體與人的AACT反應，主要用于惡性纖維組織細胞瘤等惡性腫瘤的診斷,也可用于胃癌﹑肺癌、腎癌等腫瘤的研究。 |
| 產(chǎn)品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Alpha-1-Antichymotrypsin (bs-0094R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Liver tissue lysates
Primary: Anti-Alpha-1-Antichymotrypsin (bs-0094R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Tissue/cell: Mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Alpha-1-Antichymotrypsin Polyclonal Antibody, Unconjugated (bs-0094R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Alpha-1-Antichymotrypsin Polyclonal Antibody, Unconjugated (bs-0094R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
