| 產(chǎn)品編號 | bs-0334R |
| 英文名稱 | MTLC Rabbit pAb |
| 中文名稱 | 致癌基因抗體 |
| 別 名 | myc target 1; Myc target 1; MYCT 1 protein; Myc target in myeloid cells protein 1; Myc target protein 1; 1110020B04Rik; AI225941; AI642973; FLJ21269; MGC156309; MGC156310; Mt-Mc1; MTLC; Mtmc1; MYCT1; MYCT1_HUMAN; Myct1; MTMC1. |
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Specific References (2) | bs-0334R has been referenced in 2 publications.
[IF=3.487] Zhang C et al.
MiR-429 regulates rat liver regeneration and hepatocyte proliferation by targeting JUN/MYC/BCL2/CCND1 signaling pathway.Cell Signal. 2018 Oct;50:80-89. WB ; Rat.
[IF=3.414] Li Y et al. Yin Yang Gong Ji pill is an ancient formula with antitumor activity against hepatoma cells J Ethnopharmacol. 2019 Oct 3;248:112267. WB ; Human&Mouse.
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| 研究領(lǐng)域 | 免疫學(xué) 染色質(zhì)和核信號 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MTLC: 21-100/235 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
MTLC is a 235 amino acid nuclear protein that is thought to regulate certain myc target genes. Downregulated in gastric cancer tissues, MTLC is expressed in the hepatocellular carcinoma cell line, Bel7402. Overexpression of MTLC is thought to influence tumorigenic conversion, promotion of apoptosis genomic instability and inhibition of hematopoietic differentiation. The gene encoding MTLC maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Additionally, Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map to chromosome 6. Function: May regulate certain MYC target genes, MYC seems to be a direct upstream transcriptional activator. Does not seem to significantly affect growth cell capacity. Overexpression seems to mediate many of the known phenotypic features associated with MYC, including promotion of apoptosis, alteration of morphology, enhancement of anchorage-independent growth, tumorigenic conversion, promotion of genomic instability, and inhibition of hematopoietic differentiation. Subcellular Location: Nucleus. Note=Expressed in nuclei of hepatocellular carcinoma cell line Bel7402 cells. Tissue Specificity: Down-regulated in gastric cancer tissues. Similarity: Belongs to the MYCT1 family. SWISS: Q8N699 Gene ID: 80177 Database links: Entrez Gene: 80177 Human Entrez Gene: 68632 Mouse SwissProt: Q8N699 Human SwissProt: Q8R411 Mouse Unigene: 18160 Human Unigene: 338242 Mouse Unigene: 55418 Rat 轉(zhuǎn)錄調(diào)節(jié)因子（Transcriptin Regulators） |
| 產(chǎn)品圖片 |
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti- MTLC (bs-0334R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 27 kD
Observed band size: 31 kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-MTLC Polyclonal Antibody, Unconjugated(bs-0334R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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