| 產(chǎn)品編號 | bs-0053R |
| 英文名稱 | Caspase-13 + 4 Rabbit pAb |
| 中文名稱 | 半胱胺酸蛋白酶蛋白-13抗體 |
| 別 名 | CASP-13; CASP4; Caspase 13 subunit 1; Caspase 13 subunit 2; Caspase 4; Caspase 4 subunit 1; Caspase 4, apoptosis related cysteine; CASPD_BOVIN; Caspase-13; Evolutionary related interleukin-1-beta-converting enzyme; ERICE. |
| 研究領(lǐng)域 | 細胞生物 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from bovine Caspase-13: 71-130/377 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Caspase-13 (also designated Evolutionarily related interleukin-1beta converting enzyme ; ERICE) involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. [subunit] Heterodimer of a small and a large subunit. The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. Mainly expressed in peripheral blood lymphocytes, spleen and placenta. Belongs to peptidase family C14. Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. Tissue Specificity: Mainly expressed in peripheral blood lymphocytes, spleen and placenta. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. Database links: SwissProt: O75601 Bovine
SwissProt: Q5E9C1 Bovine
Entrez Gene: 338039 Bovine Caspase-13 (也稱 Evolutionarily related interleukin-1beta convertingenzyme; ERICE) 參與活化Caspase級聯(lián)反應(yīng)，實施調(diào)亡。 它既能激活某些蛋白質(zhì),細胞死亡所必需的蛋白質(zhì)；又能使某些蛋白質(zhì)失活以使細胞能夠存活。它的異原二聚體構(gòu)成大、小兩種亞單位，這兩種亞單位來源于前體序列由一個自動催化機制而形成或由Caspase-8剪切而成。 Caspase-13主要表達于外周淋巴細胞、脾臟和胎盤。屬于肽酶家族C14成員. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
