| 產(chǎn)品編號 | bs-0215R |
| 英文名稱 | CD95/FAS Rabbit pAb |
| 中文名稱 | 載脂蛋白1抗體 |
| 別 名 | ALPS 1A; ALPS1A; APO 1; Apo-1; Apo 1 antigen; APO 1 cell surface antigen; Apo-1 antigen; APO1; Apo1 antigen; APO1 cell surface antigen; Apoptosis antigen 1; Apoptosis mediating surface antigen FAS; Apoptosis-mediating surface antigen FAS; APT 1; APT1; CD |
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Specific References (4) | bs-0215R has been referenced in 4 publications.
[IF=4.651] Ewelina Dobosz. et al. Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation. Dis Model Mech. 2021 Mar;14(3) IHC ; Mouse.
[IF=3.417] Lei Xiang. et al. Antitumor effects of curcumin on the proliferation, migration and apoptosis of human colorectal carcinoma HCT?116 cells. Oncol Rep. 2020 Nov;44(5):1997-2008 WB ; Human.
[IF=2.791] Wang Q et al. Cdc20 and molecular chaperone CCT2 and CCT5 are required for the Muscovy duck reovirus p10. 8-induced cell cycle arrest and apoptosis. Vet Microbiol. 2019 Jun 28;235:151-163. WB ; Monkey.
[IF=1.08] Zhou, Ke-Wen, et al. "Overexpression of CIRP may reduce testicular damage induced by cryptorchidism." Clinical & Investigative Medicine 32.2 (2009): E103-E111. Mouse.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=2μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | 40-50 |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat FAS: 35-110/327 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011] Function: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Subunit: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2, 3, 4, 5, 6: Secreted. Tissue Specificity: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. Post-translational modifications: N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. DISEASE: Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. Similarity: Contains 1 death domain. Contains 3 TNFR-Cys repeats. SWISS: Q63199 Gene ID: 246097 Database links: Entrez Gene: 355 Human Entrez Gene: 14102 Mouse Omim: 134637 Human SwissProt: P25445 Human SwissProt: P25446 Mouse Unigene: 244139 Human Unigene: 1626 Mouse Unigene: 162521 Rat FAS(也稱作Apo-1;CD95;FasL receptor;TNFR)是一個含有死亡結(jié)構(gòu)域的受體，是一型膜蛋白，屬于TNF-R家族成員，具有誘導(dǎo)細胞凋亡的功能，廣泛分布于許多不同類型的細胞，主要用于各種惡性腫瘤（包括：乳腺癌、腎細胞癌、胃癌、肺癌以及肝病等）的研究。分子量為：40-50kDa。 FAS的凋亡信號主要是通過與其胞漿區(qū)相關(guān)的死亡結(jié)構(gòu)域蛋白FADD介導(dǎo)的。FAS與FasL結(jié)合后，F(xiàn)ADD一方面通過C端的DD結(jié)合FAS, 另一方面通過N端的DED與Caspase-8 N端DED結(jié)合，通過Caspase-8誘導(dǎo)效應(yīng)性Caspase 蛋白酶的激活，并最終導(dǎo)致細胞凋亡的發(fā)生。FAS主要表達于活化淋巴細胞、單核細胞、中性粒細胞和成纖維細胞等。 Fas又稱作APO-1/CD95，屬TNF受體家族。Fas基因編碼產(chǎn)物為分子量45KD的跨膜蛋白，分布于胸腺細胞，激活的T和B淋巴細胞，巨噬細胞，肝、脾、肺、心、腦、腸、睪丸和卵巢細胞等。 Fas蛋白與Fas配體結(jié)合后，會激活caspase，導(dǎo)致靶細胞走向凋亡。 |
| 產(chǎn)品圖片 |
Sample: serum (Rat) at 40 ug plasma (Rat) at 40 ug Primary: Anti-CD95 (bs-0215R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 42 kD
Sample:
Lane 1: Thymus (Mouse) Lysate at 40 ug
Lane 2: Thymus (Rat) Lysate at 40 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: Raji (Human) Cell Lysate at 30 ug
Lane 5: HT1080 (Human) Cell Lysate at 30 ug
Lane 6: A431 (Human) Cell Lysate at 30 ug
Primary: Anti- CD95/FAS (bs-0215R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45/52 kD
Observed band size: 52 kD
Blank control:Raji.
Primary Antibody (green line): Rabbit Anti-CD95/FAS antibody (bs-0215R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control(blue):Mouse Kidney (fixed with 2% paraformaldehyde for 10 min at 37℃). Primary Antibody:Rabbit Anti-CD95/FAS antibody (bs-0215R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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