| 產(chǎn)品編號 | bs-0639R |
| 英文名稱 | Rabbit Anti-GFP antibody |
| 中文名稱 | 綠色熒光蛋白抗體 |
| 別 名 | Green Fluorecent protein. 綠色熒光蛋白 GFP; 綠色熒光蛋白 (GFP); |
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Specific References (2) | bs-0639R has been referenced in 2 publications.
[IF=3.811] Mou C et al. Identification of Nuclear Localization Signals in the ORF2 Protein of Porcine Circovirus Type 3. Viruses. 2019 Nov 22;11(12). pii: E1086. WB ;
[IF=0.95] Li, Z-G., et al. "Knockdown of Porcine Endogenous Retroviruses by RNA Interference in Chinese Experimental Miniature Pig Fibroblasts." Transplantation Proceedings. Vol. 45. No. 2. Elsevier, 2013. ICC ;
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| 產(chǎn)品類型 | 標簽抗體 |
| 研究領域 | 細胞生物 免疫學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Recombinant protein |
| 產(chǎn)品應用 | WB=1:20000-50000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from belt jellyfish GFP: 151-238/238 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Subunit: Monomer. Tissue Specificity: Photocytes. Post-translational modifications: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Similarity: Belongs to the GFP family. Database links:
該抗體只用于免疫組織化學、免疫熒光、酶聯(lián)免疫使用。 GFP在科學研究上有著驚人的用途，因為它能夠使我們直接看到細胞內部的運動。情況。在任何指定的時間我們都可以輕易地找出GFP在哪兒：你只需要用紫外光去照射，這時所有的GFP都將發(fā)出鮮艷的綠色。 不妨做個實驗：你把GFP連接到你有興趣觀察的任何對象上。比如，你可以把它連接到一種病毒上。然后，隨著病毒在宿主體內不斷擴散，你就可以通過跟蹤發(fā)出的綠光來觀察病毒的擴散途徑；或者你把它接合到一種蛋白質上并通過顯微鏡觀察它在細胞內部的移動。 GFP是一種現(xiàn)成的熒光蛋白質，因此它特別容易使用。大多數(shù)可以處理光的蛋白質都利用外來的分子吸收和釋放光子。例如，我們眼睛里的視紫紅質利用維生素來感光。這些“發(fā)光團”必須是專門為了發(fā)光而生成的，并且被仔細地插入到該蛋白質分子內，不同的是，GFP控制光的部位是其自身的一部分，僅由氨基酸構建而成，該部位含有一段三個氨基酸組成的特殊序列：絲氨酸 Ser－酪氨酸Tyr－甘氨酸 Gly（有時絲氨酸會被相似的蘇氨酸取代）。當?shù)鞍踪|鏈折疊時，這段短片段就被深埋在蛋白質內部，然后，發(fā)生一系列化學反應：甘氨酸與絲氨酸之間形成化學鍵，生成一個新的閉合環(huán)，隨后這個環(huán)會自動脫水。最終，經(jīng)過大約一個小時的反應，周圍環(huán)境中的的氧氣攻擊酪氨酸的一個化學鍵，形成一個新的雙鍵并合成熒光發(fā)色團。由于GFP可以形成自己的發(fā)色團，它非常適合于基因工程。你根本不必擔心操作任何奇怪的發(fā)色團，你只需要利用遺傳學的方法操縱細胞合成GFP蛋白質，GFP就會自動折疊并開始發(fā)光。 GFP的用途已經(jīng)擴展到藝術和商務領域，育種工作者正在探索利用GFP來創(chuàng)造特殊的熒光植物和各種魚類，GFP已經(jīng)被移植到大鼠、老鼠、青蛙、有翅昆蟲、蠕蟲以及不計其數(shù)的其它生物體內。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
Lane 2: Control (-) E.coli Lysate at 4 ug
Primary: Anti-GFP (bs-0639R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Sample:
GFP-Tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
Primary:
Anti-GFP (bs-0639R) at 1/20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 30 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
