| 產(chǎn)品編號 | bs-0879R |
| 英文名稱 | Rabbit Anti-FLAG Tag (CT) antibody |
| 中文名稱 | FLAG Tag標簽（C端）抗體 |
| 別 名 | DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; DYKDDDDK-Tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG-tag. |
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Specific References (4) | bs-0879R has been referenced in 4 publications.
[IF=5.714] Mengmeng Zhao. et al. Interferon inducible porcine 2′, 5′-oligoadenylate synthetase like-1 protein limits porcine reproductive and respiratory syndrome virus 2 infection via the MDA5-mediated interferon-signaling pathway. INT IMMUNOPHARMACOL. 2022 Oct;111:109151 IP, WB ; Monkey, Pig.
[IF=5.52] Zhang, Zhenfeng, et al. "Human Bocavirus NP1 Inhibits IFN-?? Production by Blocking Association of IFN Regulatory Factor 3 with IFNB Promoter."The Journal of Immunology 189.3 (2012): 1144-1153.
[IF=3.636] Mengmeng Zhao. et al. Molecular cloning and functional analysis of Macaca mulatta STING. Dev Comp Immunol. 2022 Feb;127:104296 WB ; Monkey.
[IF=3.412] Mengmeng Zhao. et al. Sequence Analysis of Macaca mulatta TRIM4 and Its Role in the Interferon Pathway. Front Vet Sci. 2022; 9: 805301 WB ; Monkey.
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| 產(chǎn)品類型 | 標簽抗體 |
| 研究領(lǐng)域 | 染色質(zhì)和核信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Recombinant protein |
| 產(chǎn)品應(yīng)用 | WB=1:5000-50000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Flag Tag peptide |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins. 蛋白質(zhì)純化的新途徑─應(yīng)用抗DYKDDDDK標簽8肽抗體檢測DYKDDDDK標簽N端8肽融合蛋白 1） DYKDDDDK標簽8肽與不同的Sepharose純化填料結(jié)合（捆綁），可不受構(gòu)象的影響，在天然或變性的條件下可進行進一步純化。 2） 可在溫和的洗脫液環(huán)境中，其結(jié)合、洗滌及洗脫步驟不會對蛋白結(jié)構(gòu)產(chǎn)生影響。 3） DYKDDDDK標簽比普通（Tag）要小得多，經(jīng)純化后的蛋白可直接進行下游操作?？捎糜谌魏伪磉_系統(tǒng)（包括： 細菌，桿狀病毒和哺乳動物） 4） DYKDDDDK8肽標簽在生理溶液pH下不帶電荷，其標簽不會干涉重組蛋白的結(jié)構(gòu)和功能，不會影響蛋白分泌 5） DYKDDDDK8肽標簽免疫原性差，重組蛋白可以不必除去標簽而直接作為抗原進行免疫。 |
| 產(chǎn)品圖片 |
Sample: Lane1:DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-FLAG Tag (bs-0879R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
