| 產(chǎn)品編號(hào) | bs-1218R |
| 英文名稱 | XRCC1 Rabbit pAb |
| 中文名稱 | X射線修復(fù)交叉互補(bǔ)蛋白抗體 |
| 別 名 | DNA repair protein XRCC1; RCC; X ray repair complementing defective repair in chinese hamster; X ray repair complementing defective repair in chinese hamster cells 1; X ray Repair Complementing Defective Repair in Chinese Hamster Cells; X ray repair cross complementing protein 1; RCC; X ray repair, complementing defective, repair in Chinese hamster; X-ray repair cross-complementing protein 1; XRCC 1; Xrcc1; XRCC1_HUMAN; XRCC 1. |
| 研究領(lǐng)域 | 腫瘤 染色質(zhì)和核信號(hào) 新陳代謝 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 69 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human XRCC1: 1-120/633 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
XRCC1 is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. In testis XRCC1 is expressed at high levels. Function: Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Subunit: Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1. Subcellular Location: Nucleus. Note=Accumulates at sites of DNA damage. Post-translational modifications: Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF. Sumoylated. Similarity: Contains 2 BRCT domains. SWISS: P18887 Gene ID: 7515 Database links: Entrez Gene: 7515 Human Entrez Gene: 22594 Mouse Omim: 194360 Human SwissProt: P18887 Human SwissProt: Q60596 Mouse Unigene: 98493 Human Unigene: 4347 Mouse Unigene: 13754 Rat XRCC-1是一種重要的DNA修復(fù)基因,在一系列內(nèi)外氧化劑(包括煙草成分)導(dǎo)致的DNA單鏈斷裂和堿基損傷修復(fù)中起到重要作用,是目前腫瘤研究的重要的蛋白。 |
| 產(chǎn)品圖片 |
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-XRCC1 Polyclonal Antibody, Unconjugated(bs-1218R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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