| 產(chǎn)品編號 | bs-1278R |
| 英文名稱 | Rabbit Anti-8-OHdG (DNA/RNA Damage) antibody |
| 中文名稱 | 8-羥基脫氧鳥苷抗體 |
| 別 名 | 8-hydroxy-2'-deoxyguanosine; 2'-Deoxy-8-oxoguanosine; 7, 8-Dihydro-8-oxo-2'-deoxyguanosine; 8-Oxo-7, 8-dihydro-2'-deoxyguanosine; 8-Oxo-7, 8-dihydrodeoxyguanosine; 2'-Deoxy-8-oxo-D-guanosine; 8-Oxo-7, 8-dihydro-2μ-deoxyguanosine, 8-Oxo-dG; 2'-Deoxy-8-hydroxyguanosine; 8-OH-脫氧鳥嘌呤; 8-羥基-2'-脫氧鳥嘌呤; 2'-脫氧-7,8-二氫-8-氧代鳥苷; 8-羥基-2-脫氧鳥苷; 8-羥基脫氧鳥苷; 2'-脫氧-8-氧代鳥嘌呤; 8-氧-2'-脫氧鳥苷; 核酸氧化8-羥基脫氧鳥苷; 8-羥基-2′-脫氧鳥苷; |
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Specific References (79) | bs-1278R has been referenced in 79 publications.
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| 產(chǎn)品類型 | 小分子抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號 表觀遺傳學(xué) Alzheimer's |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat,Species independent |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 0.283 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated 8-OHdG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer's disease. Function: Oxoguanine 8 (8-Oxoguanine) is a mutagenic oxidative damage product of guanine. Guanine is the main target for reactive oxygen species in DNA, with 8-oxoguanine being the most frequent base lesion. Thus formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1. 8-OHDG作為內(nèi)源性及外源性因素對DNA氧化損傷作用的生物標(biāo)志物，是一個極有前途的指標(biāo)，通過8-OHDG的檢測可以評估體內(nèi)氧化損傷和修復(fù)的程度，氧化應(yīng)激與DNA損傷的相互關(guān)系，對研究退行性疾病、衰老機(jī)制、癌發(fā)生機(jī)制、環(huán)境毒物與氧化應(yīng)激的關(guān)系等均有重要的意義，也可以用來評價抗氧化劑治療DNA氧化損傷的效果。已經(jīng)證實(shí)，飲食、超氧化物歧化酶、Melatonin等對8-OHDG水平具有調(diào)節(jié)作用。近期日本學(xué)者制成一種抗氧化作用雞尾酒，由葵花籽、綠茶和維生素c組成，證實(shí)了對FeC12誘導(dǎo)的8-OHDG異常增加有回調(diào)作用。隨著研究的深入，8-OHDG的應(yīng)用范圍必將越來越廣，但到目前為止，各種檢測方法都還需進(jìn)一步完善，因而深入開展這項研究工作將有十分重要的實(shí)際意義。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue(left panel was injury,Right panel was normal); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-8-OHdG Polyclonal Antibody, Unconjugated(bs-1278R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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