| 產(chǎn)品編號 | bs-1382R |
| 英文名稱 | SNAI2 Rabbit pAb |
| 中文名稱 | 鋅指轉(zhuǎn)錄因子Slug抗體 |
| 別 名 | SLUG; Neural crest transcription factor Slug; Protein snail homolog 2; Slug(chicken homolog) zinc finger protein; Slug homolog zinc finger protein; Slug zinc finger protein; SLUGH 1; SLUGH; SLUGH1; SNAI 2; SNAI-2; SNAI2_HUMAN; Snail 2; Snail homolog 2; Sn |
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Specific References (7) | bs-1382R has been referenced in 7 publications.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Slug: 5-120/268 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008]. Function: Transcriptional repressor. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occluding (OCLN) and subsequent oncogenic transformation of epithelial cells. Subunit: Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains). Subcellular Location: Nucleus. Cytoplasm. Note=Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions. Tissue Specificity: Expressed in most adult human tissues, including spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Not detected in peripheral blood leukocyte. Expressed in the dermis and in all layers of the epidermis, with high levels of expression in the basal layers (at protein level). Expressed in osteoblasts (at protein level). Expressed in mesenchymal stem cells (at protein level). Expressed in breast tumor cells (at protein level). Post-translational modifications: GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation. DISEASE: Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) . WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1 Similarity: Belongs to the snail C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers. SWISS: O43623 Gene ID: 6591 Database links: Entrez Gene: 6591 Human Entrez Gene: 20583 Mouse Entrez Gene: 432368 Chicken Omim: 602150 Human SwissProt: O43623 Human SwissProt: P97469 Mouse SwissProt: Q3UZ96 Mouse Unigene: 360174 Human Unigene: 4272 Mouse Unigene: 43117 Rat 轉(zhuǎn)錄調(diào)節(jié)因子（Transcriptin Regulators） 鋅指轉(zhuǎn)錄因子Slug主要用于消化系統(tǒng)腫瘤轉(zhuǎn)移方面的研究。 |
| 產(chǎn)品圖片 |
Sample:
Lane1: Heart (Rat) Lysate at 30 ug
Lane2: Liver (Rat) Lysate at 30 ug
Primary: Anti-SNAI2/SLUG (bs-1382R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 30kD
Observed band size : 30kD
Sample:
Lane 1: Mouse NIH/3T3 cell lysates
Lane 2: Human MDA-MB-231 cell lysates
Primary: Anti-SNAI2 (bs-1382R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 34 kDa
Sample:
Lane 1: Mouse Breast tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Human MDA-MB-231 cell lysates
Lane 4: Human U2OS cell lysates
Primary: Anti-SNAI2 (bs-1382R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 34 kDa
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SNAI2 Polyclonal Antibody, Unconjugated(bs-1382R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control（black line）:HepG2.
Primary Antibody (green line): Rabbit Anti-SNAI2 antibody (bs-1382R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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