| 產(chǎn)品編號(hào) | bs-1489R |
| 英文名稱 | BIRC6 Rabbit pAb |
| 中文名稱 | Apollon凋亡抑制蛋白抗體 |
| 別 名 | BIRC6; Apollon; Apollon protein; Baculoviral IAP repeat containing 6; Baculoviral IAP repeat containing protein 6; BIRC 6; BRUCE; FLJ13726; FLJ13786; KIAA1289; Ubiquitin conjugating BIR domain enzyme apollon; BIRC6_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 細(xì)胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 530 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Apollon C terminus: 4701-4857/4857 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Conserved genes that encode inhibitors of apoptosis proteins (IAPs) are frequently overexpressed in human cancers and are associated with resistance to therapy. IAPs contain a 70 amino acid cysteine and histidine rich baculovirus IAP repeat (BIR) and often a zinc binding RING finger. Chen et al. (1999) found that some brain cancer cell lines with high expression of BIRC6 exhibited resistance to apoptosis in the presence of anticancer drugs. Treatment of a high expressing cell line with BIRC6 antisense oligonucleotides reduced BIRC6 expression and reversed the resistance to anticancer drugs. Function: Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification. Subunit: Homodimer. Binds the activated, processed forms of CASP3, CASP6, CASP7 and CASP9. Interacts with RNF41, DIABLO/SMAC, HTRA2, KIF23/MKLP1, USP8/UBPY, BIRC5/survivin, MAP2K1/MEK1, RAB8A/RAB8, RAB11A/RAB11, PLK1, EXOC3/SEC6 and EXOC4/SEC8. Subcellular Location: Golgi apparatus, trans-Golgi network membrane. Midbody. Endosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, centrosome. Note=Concentrates in a pericentriolar compartment in interphase, moves partially to spindle poles in metaphase, and finally localizes to the spindle midzone and the midbody in telophase and during cytokinesis. Tissue Specificity: Expressed in brain cancer cells. Post-translational modifications: Ubiquitinated. Ubiquitination is mediated by the RNF41 E3 ligase and leads to proteasomal degradation, impairing inhibition of apoptosis. Deubiquitinated by USP8/UBPY. Similarity: In the C-terminal section; belongs to the ubiquitin-conjugating enzyme family. Contains 1 BIR repeat. SWISS: Q9NR09 Gene ID: 57448 Database links: Entrez Gene: 57448 Human Entrez Gene: 12211 Mouse Omim: 605638 Human SwissProt: Q9NR09 Human SwissProt: O88738 Mouse Unigene: 150107 Human Unigene: 290908 Mouse Apollon是凋亡抑制蛋白家族IAP(inhibitors of apoptosis protein 凋亡抑制因子)的成員之一，它通過抑制Smac和caspase-9酶活性而產(chǎn)生抗凋亡作用它是IAP家族中分子量最大的成員,Apollon在某些腫瘤組織中表達(dá)。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BIRC6) Polyclonal Antibody, Unconjugated (bs-1489R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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