| 產品編號 | bs-1941R |
| 英文名稱 | Zfp91 Rabbit pAb |
| 中文名稱 | 白血病相關新基因抗體 |
| 別 名 | zinc finger protein 91; FKSG11; HGNC:14983; PZF; zinc finger protein 91 homolog(mouse); zinc finger protein homologous to mouse Zfp91; ZFP91_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 染色質和核信號 神經生物學 細胞凋亡 轉錄調節(jié)因子 結合蛋白 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Zfp91: 301-400/570 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by ZFP91 is a member of the zinc finger family of proteins. This protein contains C2H2 type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins. May be involved in transcriptional regulation. May play an important role in cell proliferation and/or anti-apoptosis Found in all the examined tissues including brain, heart, kidney, lung, liver, spleen, thymus, skeletal muscle, ovary and testis. Belongs to the krueppel C2H2-type zinc-finger protein family. Function: Atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis. Subunit: Interacts with MAP3K14/NIK. Subcellular Location: Nucleus. Tissue Specificity: Expressed ubiquitously, particularly at high level in testis. Isoform 2 is testis specific. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers. SWISS: Q96JP5 Gene ID: 80829 Database links: Entrez Gene: 80829 Human Entrez Gene: 109910 Mouse SwissProt: Q96JP5 Human SwissProt: Q62511 Mouse Unigene: 524920 Human Unigene: 290924 Mouse Unigene: 1772 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Zfp91) Polyclonal Antibody, Unconjugated (bs-1941R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Zfp91) Polyclonal Antibody, Unconjugated (bs-1941R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
