| 產(chǎn)品編號 | bs-2734R |
| 英文名稱 | Bcl-6 Rabbit pAb |
| 中文名稱 | 原癌基因Bcl-6抗體 |
| 別 名 | Bcl-6/5; Bcl-6+Bcl-5; B-cell lymphoma 6 protein isoform 1; zinc finger transcription factor BCL6S; B-cell lymphoma 6 protein transcript; BCL-5; BCL6; Bcl 6; protein LAZ-3; B-cell lymphoma 5 protein; zinc finger and BTB domain-containing protein 27; lymphoma-associated zinc finger gene on chromosome 3; zinc finger protein 51; cys-his2 zinc finger transcription factor; BCL5; BCL6A; LAZ3; ZBTB27; ZNF51; BCL6_HUMAN. |
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Specific References (4) | bs-2734R has been referenced in 4 publications.
[IF=6.208] Genxi Zhang. et al. miRNA-10a-5p Targeting the BCL6 Gene Regulates Proliferation, Differentiation and Apoptosis of Chicken Myoblasts. INT J MOL SCI. 2022 Jan;23(17):9545 WB ; Chicken.
[IF=2.952] Shuang Zhang. et al. Knockdown of miR?205?5p alleviates the inflammatory response in allergic rhinitis by targeting B?cell lymphoma 6. Mol Med Rep. 2021 Nov;24(5):1-11 IF,IHC ; mouse.
[IF=2.48] Szabó, Krisztina, et al. "Expansion of circulating follicular T helper cells associates with disease severity in childhood atopic dermatitis." Immunology Letters (2017). IHC-P ; Human.
[IF=1.26] Gong, Qiaoqiao, et al. "Increased levels of CCR7 (lo) PD?1 (hi) CXCR5+ CD4+ T cells, and associated factors Bcl?6, CXCR5, IL?21 and IL?6 contribute to repeated implantation failure." Experimental and Therapeutic Medicine 14.6 (2017): 5931-5941. IHC-P ; Human.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 78 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Bcl-6: 331-430/706 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq]. Function: Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis. Subunit: Homodimer. Interacts (via BTB domain) with BCOR; the interaction is direct. Interacts (via BTB domain) with NCOR2; the interaction is direct. Interacts with ZBTB7 and BCL6B (By similarity). Interacts with the catalytic domain of HDAC9. Subcellular Location: Nucleus (By similarity). Tissue Specificity: Expressed in germinal center T and B-cells and in primary immature dendritic cells. Post-translational modifications: Phosphorylated by MAPK1 in response to antigen receptor activation at Ser-333 and Ser-343. Phosphorylation induces its degradation by ubiquitin/proteasome pathway. Polyubiquitinated. Polyubiquitination leads to degradation by the proteasome. DISEASE: Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions. Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1. Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF. Similarity: Contains 1 BTB (POZ) domain. Contains 6 C2H2-type zinc fingers. SWISS: P41182 Gene ID: 604 Database links: Entrez Gene: 604 Human Entrez Gene: 12053 Mouse Omim: 109565 Human SwissProt: P41182 Human SwissProt: P41183 Mouse Unigene: 478588 Human |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Bcl-6 polyclonal antibody, unconjugated (bs-2734R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Bcl-6) Polyclonal Antibody, Unconjugated (bs-2734R) at 1:25 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Bcl-6) Polyclonal Antibody, Unconjugated (bs-2734R) at 1:25 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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