產(chǎn)品編號 | bs-2272R |
英文名稱 | phospho-ATM (Ser1981) Rabbit pAb |
中文名稱 | 磷酸化毛細血管擴張性共濟失調(diào)癥突變蛋白抗體 |
別 名 | ATM(phospho-Ser1981); ATM(phospho S1981); AT complementation group A; AT complementation group C; AT complementation group D; AT complementation group E; AT mutated; AT protein; AT1; ATA; Ataxia telangiectasia gene mutated in human beings; Ataxia telangie |
![]() |
Specific References (2) | bs-2272R has been referenced in 2 publications.
[IF=5.008] Yin, H., et al. "The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum." Oncotarget (2016). IHC-P ; Chicken.
[IF=5.008] Guo, Hongrui, et al. "Dietary NiCl2 causes cell cycle arrest in the broiler's kidney." Oncotarget. (2015) 6.34:35964-77. IHC-P ; Chicken.
|
產(chǎn)品類型 | 磷酸化抗體 |
研究領域 | 信號轉(zhuǎn)導 細胞凋亡 細胞周期蛋白 激酶和磷酸酶 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human (predicted: Mouse,Rat) |
產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg /test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 370 kDa |
檢測分子量 | |
細胞定位 | 細胞核 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human ATM around the phosphorylation site of Ser1981: EG(p-S)QS |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation, apoptosis, and response to DNA damage repair (DNA damage caused by such things as ionizing irradiation activates ATM kinase). The C terminal region has extensive homology to the catalytic domains of Phosphatidylinositol 3 kinases (PI3 kinases).
Subcellular Location: Nucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin. Tissue Specificity: Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes. Post-translational modifications: Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase. Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60. DISEASE: Defects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation. Note=Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients. Note=Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL). Note=Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure. Similarity: Belongs to the PI3/PI4-kinase family. ATM subfamily. Contains 1 FAT domain. Contains 1 FATC domain. Contains 1 PI3K/PI4K domain. SWISS: Q13315 Gene ID: 472 Database links: Entrez Gene: 472 Human Entrez Gene: 11920 Mouse Omim: 607585 Human SwissProt: Q13315 Human SwissProt: Q62388 Mouse Unigene: 367437 Human Unigene: 5088 Mouse Unigene: 214048 Rat |
產(chǎn)品圖片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-ATM(Ser1981) Polyclonal Antibody, Unconjugated(bs-2272R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): Hela (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 20 min on ice).
Primary Antibody (green line): Rabbit Anti- phospho-ATM(Ser1981) antibody (bs-2272R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
|
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
| 91茄子视频在线观看 | 肥婆A片无套内谢WWW | 中文字幕亚洲熟妇熟色av | 少妞躁BBB少妞躁BBBB | 久久国产精品电影 | 国产裸体爆乳abb大全 | 日本理论午午夜理论片 | 69精品人人槡人妻人人玩简单 | 国产一级内射麻豆91 | 97人妻人人揉人人躁人人免费 | 四川少妇WBBBB搡BBBB嗓 | 精品91传媒成人生活A片 | 日本一级婬片A片免费看软件 | 无码人妻丰满熟妇啪啪 | 中文字幕人妻一区二区 | 成人午夜啪免费视频在线观看软件 | 农村拗女一区二区三区在线播放 | 天天日天天干天天插1 | 国内精品人妻无码久久久影院蜜桃 | 无码人妻一区二区三区蜜桃视频 | 一本大道HEYZO无码S99 | 亚洲精品乱码久久久久久皂宅 | 久久人妻熟女中文字幕av蜜芽 | 亚洲一区二区无码乱伦 | 亚洲精品无码中文字幕 | av无码在线免费观看 | 少妇人妻无套进入69va | 苍井空和黑人最猛一次 | 小向美奈子av亚洲一区 | 少妇人妻偷人精品视频蜜桃 | 成人区人妻精品一熟女 | 专干老熟女300部合集 | 黄色三级片视频网站 | 国产精品自拍第一页 | 97精品伊人久久久大香线蕉97 | 成人免费在线视频 | 古典武侠人妻另类欧美日韩 | 中文字幕免费在线观看 | 99精品欧美一区二区三区综合在线 | 午夜国产精品秘 入口无码 国产成人无码综合亚洲日韩 |