| 產(chǎn)品編號(hào) | bs-2762R |
| 英文名稱 | DARPP32 Rabbit pAb |
| 中文名稱 | 多巴胺、cAMP調(diào)節(jié)磷蛋白抗體 |
| 別 名 | DARPP 32; DARPP-32; Dopamine and cAMP regulated neuronal phosphoprotein; Dopamine and cAMP regulated phosphoprotein DARPP 32; Dopamine and cAMP regulated phosphoprotein DARPP32; FLJ20940; IPPD; Neuronal phosphoprotein DARPP 32; PPP1R1B; Protein phosphatase 1 regulatory(inhibitor) subunit 1B; Protein phosphatase 1 regulatory subunit 1B. |
| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 通道蛋白 細(xì)胞膜受體 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat,GuineaPig (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DARPP32: 51-150/204 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]. Function: Inhibitor of protein-phosphatase 1. Subcellular Location: Cytoplasm. Post-translational modifications: Phosphorylation of Thr-34 is required for activity. Similarity: Belongs to the protein phosphatase inhibitor 1 family. SWISS: Q9UD71 Gene ID: 84152 Database links: Entrez Gene: 84152 Human Entrez Gene: 19049 Mouse Omim: 604399 Human SwissProt: Q9UD71 Human SwissProt: Q60829 Mouse Unigene: 286192 Human Unigene: 45372 Mouse Unigene: 70366 Rat ????DARPP-32通過(guò)自身不同位點(diǎn)的磷酸化對(duì)蛋白質(zhì)磷酸化和去磷酸化過(guò)程發(fā)揮著雙向調(diào)控的作用.DARRP-32在腦內(nèi)存在于接受多巴胺能投射的神經(jīng)元中,其磷酸化過(guò)程受多種神經(jīng)遞質(zhì)的調(diào)控,在信號(hào)轉(zhuǎn)導(dǎo)途徑中處于中心位置,整合多種胞內(nèi)信號(hào)轉(zhuǎn)導(dǎo),調(diào)節(jié)神經(jīng)元的電學(xué)和化學(xué)性質(zhì)以及動(dòng)物的生理行為反應(yīng),參與多種生理功能和病理過(guò)程,包括藥物成癮、抑郁癥、精神分裂癥等.此外,DARPP-32在非神經(jīng)系統(tǒng)的功能也逐漸被人們所認(rèn)識(shí). ????多巴胺和cAMP調(diào)節(jié)的磷蛋白(dopamine and adenosine 3'5'-monophosphate-regulated phospho-protein,Mr 32 kD,DARPP-32)是一個(gè)具有雙重功能的獨(dú)特蛋白,它既是磷酸酶(如PP-1)的抑制劑,也是蛋白激酶(如PKA)的抑制劑。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DARPP32 Polyclonal Antibody, Unconjugated(bs-2762R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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