| 產(chǎn)品編號(hào) | bs-3506R |
| 英文名稱 | NEDD4-2 Rabbit pAb |
| 中文名稱 | 泛素連接酶NEDD4-2抗體 |
| 別 名 | NEDD4L; E3 ubiquitin protein ligase NEDD4 like protein; E3 ubiquitin-protein ligase NEDD4-like; E3 ubiquitin-protein ligase NEDD4-like protein; KIAA0439; NED4L_HUMAN; NEDD4 2; NEDD4 2c; NEDD4-2; NEDD4-2a; NEDD4.2; NEDD4b; NEDD4L; NEDD4La; NEDD4Lb; NEDD4Lc; NEDD4Ld; NEDD4Le; NEDD4Lf; NEDD4Lg; NEDD4Lh; NEDL3; Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase; Neural precursor cell expressed, developmentally down regulated 4 like; RSP5; Ubiquitin protein ligase Rsp5. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號(hào) 神經(jīng)生物學(xué) 細(xì)胞凋亡 激酶和磷酸酶 表觀遺傳學(xué) 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 112 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NEDD4L/NEDD4-2: 701-800/975 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Subunit: Interacts with UBE2E3 (By similarity). Interacts with NDFIP1 and NDFIP2 (By similarity); this interaction activates the E3 ubiquitin-protein ligase. Interacts via its WW domains with SCNN1A, SCNN1B, SCNN1G, SCN1A, SCN2A, SCN3A, SCN5A, SCN8A, SCN9A, SCN10A and CLCN5. Interacts with SMAD2, SMAD3, SMAD6 and SMAD7. The phosphorylated form interacts with 14-3-3 proteins. Interacts with Epstein-Barr virus LMP2A. Interacts with TNK2. Interacts with WNK1. Interacts with SGK1. Interacts (via C2 domain) with NPC2. Subcellular Location: Cytoplasm. Note=May be recruited to exosomes by NDFIP1. Tissue Specificity: Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. Post-translational modifications: Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation. Auto-ubiquitinated. Similarity: Contains 1 C2 domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 4 WW domains. SWISS: Q96PU5 Gene ID: 23327 Database links: Entrez Gene: 23327 Human Entrez Gene: 83814 Mouse Entrez Gene: 399396 Xenopus laevis Omim: 606384 Human SwissProt: Q96PU5 Human SwissProt: Q8CFI0 Mouse Unigene: 185677 Human Unigene: 98668 Mouse Unigene: 72824 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NEDD4L Polyclonal Antibody, Unconjugated(bs-3506R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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