| 產(chǎn)品編號(hào) | bs-3720R |
| 英文名稱 | phospho-eIF4EBP1 (Ser65 + Thr70) Rabbit pAb |
| 中文名稱 | 磷酸化eIF4E結(jié)合蛋白抗體 |
| 別 名 | eIF4EBP1(phospho S65/Th70); eIF4EBP1(phospho Ser65/Thr70); p-eIF4EBP1(Ser65/Thr70); Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; phosphorylated heat-and acid-stable protein regulated by insulin 1(PHAS-I); 4EBP1_HUMAN. |
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Specific References (1) | bs-3720R has been referenced in 1 publications.
[IF=4.486] Han Zhang. et al. m6A methyltransferase METTL3 promotes retinoblastoma progression via PI3K/AKT/mTOR pathway. J Cell Mol Med. 2020 Nov;24(21):12368-12378 WB ; Human.
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| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 13 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from mouse 4EBP1 around the phosphorylation site of Ser65/Thr70: RN(p-S)PVTK(p-T)PP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]. Function: Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. Subunit: Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts with RPTOR. Post-translational modifications: Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Similarity: Belongs to the eIF4E-binding protein family. SWISS: Q60876 Gene ID: 13685 Database links: Entrez Gene: 1978 Human Entrez Gene: 13685 Mouse Omim: 602223 Human SwissProt: Q13541 Human SwissProt: Q60876 Mouse Unigene: 411641 Human Unigene: 6700 Mouse Unigene: 11161 Rat 4E-BP1是一種分子量為10-12kDa 的酸性蛋白，與eIF4G 競(jìng)爭結(jié)合。4E-BPs 對(duì) eIF4E 的結(jié)合是可逆的，并依賴于 4E-BP 的磷酸化狀態(tài)。非磷酸化的4E-BP1 能與 eiF4E 牢固結(jié)合，而磷酸化的4E-BP1則不能。 而一些蛋白激酶Akt、 TOR、 MAPK、S6激酶和Cdc2是已知的能通過對(duì)35、45、69位的蘇氨酸殘基或64位的絲氨酸殘基進(jìn)行磷酸化而使4E-BP1喪失對(duì)eIF4E結(jié)合能力的激酶。然而，并非所有的磷酸化事件都能同樣地阻斷4EBP1-eIF4E的相互作用。 |
