| 產(chǎn)品編號 | bs-3658R |
| 英文名稱 | NIPP1 Rabbit pAb |
| 中文名稱 | 核抑制蛋白磷酸酶1抗體 |
| 別 名 | Activator of RNA decay; ARD 1; ARD1; Homolog of E.coli RNase E; NIPP 1; NIPP1; Nuclear inhibitor of protein phosphatase 1; Nuclear subunit of PP1; PPP1R8; Protein phosphatase 1 regulatory inhibitor subunit 8; Protein phosphatase 1 regulatory subunit 8; RN |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Zebrafish (predicted: Human,Rat,Rabbit,Pig,Cow,Chicken,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NIPP1: 21-120/351 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Inhibitor subunit of the major nuclear protein phosphatase 1 (PP1). It has RNA binding activity but does not cleave RNA and may target PP1 to RNA associated substrates. May also be involved in pre mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Function: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Subunit: Interacts with phosphorylated CDC5L, SF3B1 and MELK. Interacts with EED, in a nucleic acid-stimulated manner. Part of a complex consisting of PPP1R8, EED, HDAC2 and PP-1. Part of the spliceosome. Interacts with PPP1CA, PPP1CB and PPP1CC. Subcellular Location: Nucleus. Nucleus speckle. Note=Primarily, but not exclusively, nuclear. Isoform Gamma: Cytoplasm. Note=Found mainly in the cytoplasm. Tissue Specificity: Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells. Post-translational modifications: May be inactivated by phosphorylation on Ser-199 or Ser-204. Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA. Similarity: Contains 1 FHA domain. SWISS: Q12972 Gene ID: 5511 Database links: Entrez Gene: 5511 Human Entrez Gene: 100336 Mouse Omim: 602636 Human SwissProt: Q12972 Human SwissProt: Q8R3G1 Mouse Unigene: 533474 Human Unigene: 105230 Mouse Unigene: 16580 Rat NIPP1是一種普遍存在于細(xì)胞核中的蛋白，它在剪接體的裝配中發(fā)揮著重要作用，主要體現(xiàn)在細(xì)胞的分化，增值及遷移中。經(jīng)研究，目前NIPP1在乳腺腫瘤、前列腺癌,以及肺癌中有較高頻率的表達,其中乳腺腫瘤中的表達頻率最高,達到70%,遠(yuǎn)高于其他腫瘤組織。表明ARD1蛋白的高表達可能是乳腺腫瘤組織的一個標(biāo)志。 |
| 產(chǎn)品圖片 |
Sample:
Placenta (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti- NIPP1 (bs-3658R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
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