| 產(chǎn)品編號(hào) | bs-3897R |
| 英文名稱(chēng) | TXNIP Rabbit pAb |
| 中文名稱(chēng) | 硫氧還蛋白結(jié)合蛋白2抗體 |
| 別 名 | ; THIF; thioredoxin binding protein 2; thioredoxin interacting protein; Thioredoxin-binding protein 2; Thioredoxin-interacting protein; upregulated by 1, 25-dihydroxyvitamin D-3; VDUP1; Vitamin D3 up-regulated protein 1; TXNIP_HUMAN. |
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Specific References (2) | bs-3897R has been referenced in 2 publications.
[IF=2.9] Li, Xiao-Feng, et al. "MicroRNA-20a negatively regulates expression of NLRP3-inflammasome by targeting TXNIP in adjuvant-induced arthritis fibroblast-like synoviocytes." Joint Bone Spine (2016). WB ; Human.
[IF=2.86] Liu, Zhenning, et al. "Silymarin attenuated paraquat-induced cytotoxicity in macrophage by regulating Trx/TXNIP complex, inhibiting NLRP3 inflammasome activation and apoptosis." Toxicology in Vitro (2017). WB, IF(ICC) ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Pig,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TXNIP: 101-200/391 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
TXNIP may act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. It interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. It functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. It is required for the maturation of natural killer cells (from SwissProt). Function: May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1). Subunit: Interacts with TXN/thioredoxin through its redox-active site. Interacts with transcriptional repressors ZBTB16, ZBTB32 and HDAC1. Interacts (via C-terminus) with ITCH (via WW domains). Interacts with DDIT4. Subcellular Location: Cytoplasm Post-translational modifications: Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation. Similarity: Belongs to the arrestin family. SWISS: Q9H3M7 Gene ID: 10628 Database links: UniProtKB/Swiss-Prot: Q9H3M7.1 硫氧還蛋白結(jié)合蛋白-2 (TRX-binding protein-2，TBP-2)又稱(chēng):維生素D3上調(diào)蛋白1(Vitamin D3 up-regulated protein 1，VDUP1)或稱(chēng)硫氧還蛋白相互作用蛋白(Thioredoxin-interacting protein，Txnip)。 TBP-2與硫氧還蛋白(thioredoxin，TRX)的活性部位結(jié)合，抑制其活性，除了參與多種氧化應(yīng)激反應(yīng)，還具有多種生物學(xué)功能。 有學(xué)者認(rèn)為：TBP-2與腫瘤密切相關(guān)，有些腫瘤組織中，TBP-2表達(dá)降低或缺失。TBP-2表達(dá)增高時(shí)，細(xì)胞周期停滯，腫瘤細(xì)胞繁殖受到抑制。目前已成為腫瘤研究的重要靶點(diǎn)。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TXNIP Polyclonal Antibody, Unconjugated(bs-3897R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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