| 產(chǎn)品編號 | bs-2367R |
| 英文名稱 | ganglioside GM1 Rabbit pAb |
| 中文名稱 | 神經(jīng)節(jié)苷酯抗體 |
| 別 名 | GM1 ganglioside; Monosialoanglioside; ganglioside GM1. 神經(jīng)節(jié)苷脂; |
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Specific References (1) | bs-2367R has been referenced in 1 publications.
[IF=11.467] Melani Solomon. et al. Altered blood-brain barrier transport of nanotherapeutics in lysosomal storage diseases. J CONTROL RELEASE. 2022 Sep;349:1031 FC ; Human.
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| 產(chǎn)品類型 | 小分子抗體 |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 細(xì)胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 1.57 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated ganglioside GM1 purified from pig |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
A member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorilation and synaptic function. Subcellular Location: Plasma membrane 含有唾液酸的鞘脂類?？藗惪耍‥.Klenk，1935）首先發(fā)現(xiàn)在患Tay－sachs病（泰薩氏幼年型黑白癡?。┑男耗X中有蓄積（Tag－Sachs gangli-oside），在腦灰白質(zhì)中含量很多，所以對這類糖脂命名為神經(jīng)節(jié)苷脂。已知糖部分是由己糖、氨基糖、唾液酸組成的腦神經(jīng)節(jié)苷脂有8種以上。由山川民夫（1951）在馬血球基質(zhì)中找到的非神經(jīng)系統(tǒng)的神經(jīng)節(jié)苷脂——主要是血苷脂[hematoside，結(jié)構(gòu)為神經(jīng)酰胺（1←1β）GLc（4←1β）Gal（3←2α）唾液酸]。神經(jīng)節(jié)苷脂較多地存在于細(xì)胞膜，以其與膜功能的關(guān)系被引起重視。破傷風(fēng)菌和霍亂菌的外毒素可與特定的神經(jīng)節(jié)苷脂分子特異性結(jié)合。在水和有機(jī)溶劑中可溶。在水中是以數(shù)百萬個分子聚合的微團(tuán)狀態(tài)存在。 神經(jīng)節(jié)苷脂對神經(jīng)再生有重大促進(jìn)作用。加拿大金爵集團(tuán)腦科學(xué)研究室首席科學(xué)家Gatte Walker博士進(jìn)一步研究發(fā)現(xiàn)，神經(jīng)生長因子（NGF）和神經(jīng)節(jié)苷脂（GM）是腦神經(jīng)再生發(fā)育必需物質(zhì)，GM介導(dǎo)NGF數(shù)十倍增強(qiáng)其活性，形成新的豐富的神經(jīng)網(wǎng)絡(luò)，修復(fù)并促使腦神經(jīng)再次發(fā)育。 GM能夠介導(dǎo)神經(jīng)生長因子NGF促進(jìn)腦神經(jīng)細(xì)胞再生，表現(xiàn)出良好的生物效應(yīng)?！段靼步煌ㄡt(yī)科大學(xué)學(xué)報》。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GM1(GS) Polyclonal Antibody, Unconjugated(bs-2367R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:RSC96 (blue). Primary Antibody: Rabbit Anti-ganglioside GM1/AF647 Conjugated antibody (bs-2367R-AF647), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
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