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Goat Anti-Mouse IgG H&L antibody (bs-0296G-HRP)

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說明書： 100ul 1ml

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產品編號	bs-0296G-HRP
英文名稱	Goat Anti-Mouse IgG H&L antibody
中文名稱	辣根過氧化物酶標記的羊抗小鼠IgG H&L
別名	Goat Anti-Mouse IgG H&L (HRP); Immunoglobulin G;
	Specific References (306) \| bs-0296G-HRP has been referenced in 306 publications. [IF=19.568] Jinman Kim. et al. Furin cleavage is required for swine acute diarrhea syndrome coronavirus spike protein-mediated cell–cell fusion. EMERG MICROBES INFEC. 2022 Aug 17 WB ; Human. PubMed:35976165 [IF=16.874] Bingcheng Yi. et al. Step-wise CAG@PLys@PDA-Cu2+ modification on micropatterned nanofibers for programmed endothelial healing. BIOACT MATER. 2022 Jul;: IHC ; Human. PubMed:10.1016/j.bioactmat.2022.07.010 [IF=16.744] Fumei He. et al. Black phosphorus nanosheets suppress oxidative damage of stem cells for improved neurological recovery. CHEM ENG J. 2023 Jan;451:138737 IHC ; Rat. PubMed:10.1016/j.cej.2022.138737 [IF=13.391] Haitao Wang. et al. A plant virus hijacks phosphatidylinositol-3,5-bisphosphate to escape autophagic degradation in its insect vector. AUTOPHAGY. 2022 Sep 10 ELISA ; Laodelphax striatellus,Insect. PubMed:36093594 [IF=12.336] Fuyan Wang. et al. Decellularized porcine cornea-derived hydrogels for the regeneration of epithelium and stroma in focal corneal defects. Ocul Surf. 2020 Oct;18:748 WB ; Human. PubMed:32841745 [IF=11.556] Wen-juan Jiang. et al. Tubular epithelial cell-to-macrophage communication forms a negative feedback loop via extracellular vesicle transfer to promote renal inflammation and apoptosis in diabetic nephropathy. Theranostics. 2022; 12(1): 324–339 WB ; Mouse,Human. PubMed:34987648 [IF=10.787] Xiaozhen Dai. et al. Nrf2 transcriptional upregulation of IDH2 to tune mitochondrial dynamics and rescue angiogenic function of diabetic EPCs. REDOX BIOL. 2022 Oct;56:102449 WB ; Mouse. PubMed:36063728 [IF=10.103] Jia Qi Liang. et al. miRNAs derived from milk small extracellular vesicles inhibit porcine epidemic diarrhea virus infection. ANTIVIR RES. 2023 Mar;:105579 WB ; Pig. PubMed:36907442 [IF=10.041] Bingcheng Yi. et al. Lysine-doped polydopamine coating enhances antithrombogenicity and endothelialization of an electrospun aligned fibrous vascular graft. Appl Mater Today. 2021 Dec;25:101198 other ; Rabbit . PubMed:10.1016/j.apmt.2021.101198 [IF=9.587] Xia, Pengcheng. et al. MicroRNA-22-3p ameliorates Alzheimer’s disease by targeting SOX9 through the NF-κB signaling pathway in the hippocampus. J NEUROINFLAMM. 2022 Dec;19(1):1-19 IHC ; Mouse. PubMed:35821145 [IF=9.221] Rui Chen. et al. Tyramine signal amplification on polystyrene microspheres for highly sensitive quantification of Aflatoxin B1 in peanut samples. SENSOR ACTUAT B-CHEM. 2022 Dec;:133120 Peanut. PubMed:10.1016/j.snb.2022.133120 [IF=9.038] Junpeng Zhao. et al. Horseradish peroxidase-catalyzed formation of polydopamine for ultra-sensitive magnetic relaxation sensing of aflatoxin B1. J Hazard Mater. 2021 Jun;:126403 ELISA ; PubMed:10.1016/j.jhazmat.2021.126403 [IF=8.786] Wuyi Zeng. et al. A Novel PD-L1-Containing MSLN Targeting Vaccine for Lung Cancer Immunotherapy. FRONT IMMUNOL. 2022; 13: 925217 ELISA ; Mouse. PubMed:35795680 [IF=8.724] Qing Ma. et al. Durable endothelium-mimicking coating for surface bioengineering cardiovascular stents. Bioact Mater. 2021 Dec;6:4786 Other ; PubMed:34095629 [IF=8.44] Shi Xiaobo. et al. miR-4443 promotes radiation resistance of esophageal squamous cell carcinoma via targeting PTPRJ. J TRANSL MED. 2022 Dec;20(1):1-14 WB ; Human. PubMed:36578050 [IF=8.355] Shi S et al. Homologous-targeting biomimetic nanoparticles for photothermal therapy and Nrf2-siRNA amplified photodynamic therapy against oral tongue squamous cell carcinoma. Chemical Engineering Journal, 2020, 124268. WB ; Mouse. PubMed:doi:10.1016/j.cej.2020.124268 [IF=8.101] Shuang Xie. et al. Indispensable role of melatonin, a scavenger of reactive oxygen species (ROS), in the protective effect of Akkermansia muciniphila in cadmium-induced intestinal mucosal damage. FREE RADICAL BIO MED. 2022 Nov;193:447 WB ; Mouse. PubMed:36328351 [IF=8.039] Xin-Chen Jiang. et al. Discovery of a Novel G-Quadruplex and Histone Deacetylase (HDAC) Dual-Targeting Agent for the Treatment of Triple-Negative Breast Cancer. J MED CHEM. 2022;XXXX(XXX):XXX-XXX WB ; Mouse, Human. PubMed:36053318 [IF=8.025] Ziyu Liu. et al. Exosomes from LSD1 knockdown breast cancer cells activate osteoclastogenesis and inhibit osteoblastogenesis. INT J BIOL MACROMOL. 2023 Feb;:123792 WB ; Human,Mouse. PubMed:36828097 [IF=8.022] Aixin Ni. et al. Degradation of amyloid β-peptides catalyzed by nattokinase in vivo and in vitro. FOOD SCI HUM WELL. 2023 Sep;12:1905 WB ; Escherichia coli. PubMed:10.1016/j.fshw.2023.02.042 [IF=7.701] Tian, Hongwei. et al. Centromeric protein K (CENPK) promotes gastric cancer proliferation and migration via interacting with XRCC5. GASTRIC CANCER. 2022 Jun;:1-17 WB ; Human. PubMed:35715658 [IF=7.666] Chaoyue Zhong. et al. Establishment of a Spermatogonial Stem Cell Line with Potential of Meiosis in a Hermaphroditic Fish, Epinephelus coioides. CELLS-BASEL. 2022 Jan;11(18):2868 WB, IF ; Fish. PubMed:36139441 [IF=7.561] Niu H. et al. Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice.. Front Immunol. 2021 Dec;12:800965-800965 WB ; Recombinant L. plantarum. PubMed:34925386 [IF=7.425] Xiaobo Hu. et al. Polydopamine-mediated quantity-based magnetic relaxation sensing for the rapid and sensitive detection of chloramphenicol in fish samples. FOOD RES INT. 2022 Sep;:111919 Other ; Other. PubMed:10.1016/j.foodres.2022.111919 [IF=7.419] Xueqi Chen. et al. GYY4137, a H2S donor, ameliorates kidney injuries in diabetic mice by modifying renal ROS-associated enzymes. BIOMED PHARMACOTHER. 2023 Jun;162:114694 WB ; Mouse. PubMed:37054540 [IF=7.419] Qin Zhang. et al. Relief of ovalbumin-induced airway remodeling by the glycyl-l-histidyl-l-lysine-Cu2+ tripeptide complex via activation of SIRT1 in airway epithelial cells. BIOMED PHARMACOTHER. 2023 Aug;164:114936 WB ; Mouse,Human. PubMed:37257226 [IF=7.333] Homogenous Magneto-Fluorescent Nanosensor for Tumor-Derived Exosomes Isolation and Analysiset al. Homogenous Magneto-Fluorescent Nanosensor for Tumor-Derived Exosomes Isolation and Analysis. ACS Sens . 2020 Jul 24;5(7):2052-2060. FCM ; Human. PubMed:32594744 [IF=7.171] Yawen Bu. et al. YLMY Tyrosine Residue within the Cytoplasmic Tail of Newcastle Disease Virus Fusion Protein Regulates Its Surface Expression to Modulate Viral Budding and Pathogenicity \| Microbiology Spectrum. Microbiol Spectr. 2021 Dec;: WB ; Hamster. PubMed:34937182 [IF=7.169] Ma, Yan-hong. et al. Inhibiting the transcription and replication of Ebola viruses by disrupting the nucleoprotein and VP30 protein interaction with small molecules. ACTA PHARMACOL SIN. 2023 Feb;:1-13 WB ; Human. PubMed:36759643 [IF=7.003] Chen et al. A novel Meloidogyne graminicola effector, MgGPP, is secreted into host cells and undergoes glycosylation in concert with proteolysis to suppress plant defenses and promote parasitism. (2017) PLoS.Patho. 13:e1006301 WB ; Mouse. PubMed:28403192
抗體來源	Goat
克隆類型	Polyclonal
交叉反應	Mouse
產品應用	WB=1:2000-20000,IHC-P=1:200-1000,IHC-F=1:200-1000,ELISA=1:2000-20000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	kDa
檢測分子量
性狀	Liquid
濃度	2.0 mg/ml
免疫原	Native Mouse IgG
亞型	IgG
純化方法	affinity purified by Protein G, nonspecific adsorbed
緩沖液	10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
保存條件	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品介紹	Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
產品圖片	Sample: Lane 1,3,5 : Rat Cerebrum tissue lysates Lane 2,4,6 : Human MCF-7 cell lysates Primary: Anti- GAPDH (bsm-33033M) at 1/5000 dilution Secondary: Goat Anti-Mouse IgG H&L-HRP (bs-0296G-HRP) at 1/20000~1/100000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA(Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33035M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining. Immobilized mouse IgG (bs-0296P, nothing else) at 0.2μg/well can bind Goat Anti-Mouse IgG H&L/HRP antibody (Cat. No. bs-0296G-HRP).

	1、抗體溶解方法
	2、抗體修復方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關于肽鏈的設計
	9、多肽的溶解與保存
	10、酶標抗體效價測定程序