| 產(chǎn)品編號 | bs-7884R |
| 英文名稱 | Cyclin B3 Rabbit pAb |
| 中文名稱 | 周期素B3抗體 |
| 別 名 | CCNB 3; CCNB3; CYCB3; G2/mitotic specific cyclin B3. |
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Specific References (1) | bs-7884R has been referenced in 1 publications.
[IF=10.75] Tie-Gang Meng. et al. Maternal EHMT2 is essential for homologous chromosome segregation by regulating Cyclin B3 transcription in oocyte meiosis. INT J BIOL SCI. 2022 Jul 11;18(11):4513-4531 IHC ; Mouse.
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| 研究領(lǐng)域 | 細胞生物 染色質(zhì)和核信號 細胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=3ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 158 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cyclin B3: 1117-1200/1395 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene belongs to the highlyconserved cyclin family, whose members are characterized by adramatic periodicity in protein abundance through the cell cycle.Cyclins function as positive regulators of cyclin-dependent kinases(CDKs), and thereby play an essential role in the control of thecell cycle. Different cyclins exhibit distinct expression anddegradation patterns, which contribute to the temporal coordinationof each mitotic event. Studies of similar genes in chicken anddrosophila suggest that this cyclin may associate with CDC2 andCDK2 kinases, and may be required for proper spindle reorganizationand restoration of the interphase nucleus. Alternatively splicedtranscript variants encoding different isoforms have been describedfor this gene. Function: Cyclins are positive regulatory subunits of thecyclin-dependent kinases (CDKs), and thereby play an essential rolein the control of the cell cycle, notably via their destructionduring cell division. Its tissue specificity suggest that it may berequired during early meiotic prophase I. Subunit: Interacts with CDK2 kinase. Subcellular Location: Nucleus. Tissue Specificity: Testis specific. In testis, it is expressed indeveloping germ cells, but not in Leydig cells. Weakly or notexpressed in other tissues. Post-translational modifications: Ubiquitinated (Probable). Ubiquitination leads to itsdegradation during anaphase entry, after degradation of CCNB1. Similarity: Belongs to the cyclin family. Cyclin AB subfamily. SWISS: Q8WWL7 Gene ID: 85417 Database links: UniProtKB/Swiss-Prot: Q8WWL7.2 |
| 產(chǎn)品圖片 |
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cyclin B3 Polyclonal Antibody, Unconjugated(bs-7884R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Cyclin B3 antibody (bs-7884R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
