| 產(chǎn)品編號(hào) | bs-9823R |
| 英文名稱 | C3orf15 Rabbit pAb |
| 中文名稱 | 3號(hào)染色體開放閱讀框15抗體 |
| 別 名 | AAT 1; AAT-1; AAT1; AAT1 alpha; AAT1_HUMAN; AAT1alpha; AMY 1 associating protein expressed in testis 1; AMY-1-associating protein expressed in testis 1; Chromosome 3 open reading frame 15; MYCBP binding protein. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 細(xì)胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 71 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AAT1/C3orf15: 131-230/603 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The c-Myc proto-oncogene plays a significant role in cell proliferation, differentiation, transformation and apoptosis. A novel c-Myc binding protein, MYCBP (also designated AMY-1), binds to the transactivation domain of c-Myc and stimulates the activation of E-box-dependent transcription. MYCBP translocates from the cytoplasm to the nucleus during S phase when increased expression of c-Myc occurs. MYCBP and AAT-1 (AMY-1-associating protein expressed in testis 1) have been shown to associate with AKAP 149 and AKAP 84 in mitochondria of somatic cells and sperm, which suggests a role for MYCBP and AAT-1 in spermatogenesis. Expression of the AAT-1 gene is regulated by two different promoters, which result in various isoforms. One promoter generates expression of the AAT-1, AAT-1å, AAT-1∫ and AAT-1© isoforms, which are specifically expressed in testis, while the other promoter generates AAT-1L, AAT-1M and AAT-1S, which are differentially expressed. Function: Isoform 4 may play a role in spermatogenesis. Subunit: Interacts with MYCBP and AKAP1. Isoform 4 is part of a complex containing MYCBP, AKAP1 and PRKAR2B. Isoform 3 does not interact with MYCBP. Subcellular Location: Cytoplasm. Mitochondrion. Isoform 4 is found in the mitochondria and localized in the neck of the sperm. Tissue Specificity: Isoform 1 is strongly expressed in the liver. Isoform 4 is widely expressed, but strongly expressed in all spermatogenesis-related tissues, including the testis, the epithelium of cauda and the corpus epididymis. Isoform 4 is present in the spermatid and mature sperm. Isoform 4 is also expressed in Leydig cells. Isoform 3 is expressed in the testis. Similarity: Isoform 4 is phosphorylated by PKA. SWISS: Q7Z4T9 Gene ID: 89876 Database links: Entrez Gene: 89876 Human Omim: 609910 Human SwissProt: Q7Z4T9 Human Unigene: 341906 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAATS1) Polyclonal Antibody, Unconjugated (bs-9823R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining
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