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PCDH20 Rabbit pAb (bs-11113R)  
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產(chǎn)品編號 bs-11113R
英文名稱 PCDH20 Rabbit pAb
中文名稱 原鈣粘附蛋白20抗體
別    名 PCD20_HUMAN; PCDH 13; PCDH 20; PCDH13; PCDH20; Protocadherin 13; Protocadherin 20; Protocadherin-13; Protocadherin-20; Protocadherin13; Protocadherin20.  
Specific References  (1)     |     bs-11113R has been referenced in 1 publications.
[IF=4.011] Hirose F et al. Expression of protocadherin-20 in mouse taste buds. Sci Rep. 2020 Feb 6;10(1):2051.  IHF-F ;  Mouse.  
研究領域 神經(jīng)生物學  信號轉(zhuǎn)導  細胞粘附分子  細胞骨架  細胞外基質(zhì)  細胞膜蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Horse)
產(chǎn)品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 98 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PCDH20: 611-710/924 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 As a subfamily of the cadherin superfamily, protocadherins are cadherin-like cell adhesion proteins that contain up to seven extracellular domains and are predominantly expressed in the nervous system. Importantly, the adhesion mechanism of protocadherins is distinct from classic cadherins. Through inactivation or overexpression, several protocadherins have been implicated in a variety of cancers. Protocadherin-20 (PCDH20), also known as protocadherin-13, is a 924 amino acid protein containing 6 cadherin domains and potentially functioning as a calcium-dependent cell-adhesion protein. In non-small cell lung cancer cell lines, a homozygous loss of PCDH20 was identified through either deletion of one allele and methylation of the other or methylation of both alleles. Hypermethylation of PCDH20 is associated with worse prognosis and clinical outcome, suggesting that PCDH20 may function as a tumor suppressor.

Function:
Potential calcium-dependent cell-adhesion protein.

Subcellular Location:
Cell membrane.

Similarity:
Contains 6 cadherin domains.

SWISS:
Q8N6Y1

Gene ID:
64881

Database links:

Entrez Gene: 64881 Human

Omim: 614449 Human

SwissProt: Q8N6Y1 Human

Unigene: 391781 Human



產(chǎn)品圖片
Sample: Lane 1: Human SH-SY5Y cell lysates Lane 2: Human U251 cell lysates Lane 3: Human U87MG cell lysates Primary: Anti-PCDH20 (bs-11113R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kDa Observed band size: 102 kDa
Sample: Hippocampus (Mouse) Lysate at 40 ug Olfactory bulb (Mouse) Lysate at 40 ug Primary: Anti-PCDH20 (bs-11113R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kD Observed band size: 100 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SV2B) Polyclonal Antibody, Unconjugated (bs-11113R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- PCDH20 antibody(bs-11113R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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