| 產(chǎn)品編號 | bs-8717R |
| 英文名稱 | SLC30A3 Rabbit pAb |
| 中文名稱 | 溶質(zhì)載體鋅轉(zhuǎn)運3抗體 |
| 別 名 | SLC30A3; Solute carrier family 30(zinc transporter), member 3; Solute carrier family 30 member 3; Zinc transporter 3; Zinc transporter ZnT 3; ZnT-3; ZNT3; ZNT3_HUMAN; OTTHUMP00000201041. |
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Specific References (1) | bs-8717R has been referenced in 1 publications.
[IF=7.419] Jinfeng Shang. et al. Chrysin protects against cerebral ischemia-reperfusion injury in hippocampus via restraining oxidative stress and transition elements. BIOMED PHARMACOTHER. 2023 May;161:114534 IHC ; Rat.
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| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Pig,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLC30A3/ZNT3: 21-120/388 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Zinc, an essential element required for cell proliferation and differentiation, plays a role in a diverse array of cellular functions, including acting as a cofactor for numerous enzymes and transcription factors and as a neuroregulator. The zinc transporter (ZnT) family regulates the supply of zinc within cells, and its members are characterized by containing six membrane-spanning domains, a large histidine-rich intracellular loop, and a C-terminal tail. ZnT-3 mediates the uptake of zinc into vesicles in brain and testis. Function: Involved in accumulation of zinc in synaptic vesicles. Subunit: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. Subcellular Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein (Probable). Similarity: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. SWISS: Q99726 Gene ID: 7781 Database links: Entrez Gene: 7781 Human Entrez Gene: 22784 Mouse Omim: 602878 Human SwissProt: Q99726 Human SwissProt: P97441 Mouse Unigene: 467981 Human Unigene: 1396 Mouse Unigene: 9297 Rat |
| 產(chǎn)品圖片 |
Positive sample：Paraformaldehyde-fixed, paraffin embedded (mouse brain);
Negative sample：Paraformaldehyde-fixed, paraffin embedded (rat intestine);
Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC30A3) Polyclonal Antibody, Unconjugated (bs-8717R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SLC30A3 Polyclonal Antibody, Unconjugated(bs-8717R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
