| 產(chǎn)品編號 | bs-2256R |
| 英文名稱 | Albumin Rabbit pAb |
| 中文名稱 | 白蛋白抗體 |
| 別 名 | Alb 1; ALB; ALBU_MOUSE; Albumin 1; Mouse serum albumin; DKFZp779N1935; PRO0883; PRO0903; PRO1341; PRO2044; PRO2619; Serum albumin. |
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Specific References (2) | bs-2256R has been referenced in 2 publications.
[IF=9.42] Aragon, Mario J., et al. "Serum-borne bioactivity caused by pulmonary multiwalled carbon nanotubes induces neuroinflammation via blood–brain barrier impairment." Proceedings of the National Academy of Sciences (2017): 201616070. IHC-F ; Mouse.
[IF=4.849] Scieszka David. et al. Neuroinflammatory and neurometabolomic consequences from inhaled wildfire smoke-derived particulate matter in the Western United States. Toxicol Sci. 2021 Dec;: IF ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 發(fā)育生物學(xué) 干細胞 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Mouse serum albumin purified from mouse serum: full length |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]. Function: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Subcellular Location: Secreted. Tissue Specificity: Plasma. Post-translational modifications: Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606. Glycated in diabetic patients. Phosphorylation sites are present in the extracellular medium. Acetylated on Lys-223 by acetylsalicylic acid. DISEASE: Familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]: Form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. Database links: Entrez Gene: 100379232 Guinea pig Entrez Gene: 100034206 Horse Entrez Gene: 213 Human Entrez Gene: 11657 Mouse Entrez Gene: 100009195 Rabbit Omim: 103600 Human SwissProt: P19121 Chicken SwissProt: P35747 Horse SwissProt: P02768 Human SwissProt: P07724 Mouse SwissProt: P49065 Rabbit Unigene: 418167 Human Unigene: 16773 Mouse Unigene: 202968 Rat |
| 產(chǎn)品圖片 |
Sample:
Lung (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Mouse serum albumin (bs-2256R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Mouse serum albumin (bs-2256R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
Sample:
Lane 1: Mouse Serum
Lane 2: Human Serum
Primary: Anti-Albumin (bs-2256R) at 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 62 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse Liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mouse serum albumin ) Polyclonal Antibody, Unconjugated (bs-2256R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mouse serum albumin) Polyclonal Antibody, Unconjugated (bs-2256R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
