| 產(chǎn)品編號 | bs-10074R |
| 英文名稱 | MUC13 Rabbit pAb |
| 中文名稱 | 粘蛋白13抗體 |
| 別 名 | MUC13 Down regulated in colon cancer 1; DRCC1; MUC 13; MUC-13; Mucin 13, cell surface associated; RECC; UNQ6194; MUC13_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 信號轉(zhuǎn)導 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Pig,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MUC13: 175-270/511 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
MUC13 is a mucin. These are secreted or transmembrane glycoproteins that are expressed mainly in the digestive tract and on epithelial surfaces. MUC13 is upregulated in gastric cancer. Mucins have also been implicated in inflammatory bowel diseases. Function: Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling. Subunit: Homodimer of beta subunits. Subcellular Location: Cell membrane; Single-pass type I membrane protein (Potential). Secreted. Note=Also exists as a soluble form. Tissue Specificity: Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach. Post-translational modifications: Cleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homo-oligomerization into dimers is dependent on intrachain disulfide bonds. Highly N-glycosylated. Similarity: Contains 3 EGF-like domains. Contains 1 SEA domain. SWISS: Q9H3R2 Gene ID: 56667 Database links: Entrez Gene: 56667 Human Omim: 612181 Human SwissProt: Q9H3R2 Human Unigene: 5940 Human |
| 產(chǎn)品圖片 |
Sample:Kidney (Mouse)Lysate at 40 ug
Primary: Anti- MUC13(bs-10074R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 54kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUC13) Polyclonal Antibody, Unconjugated (bs-10074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
