| 產(chǎn)品編號(hào) | bs-13187R |
| 英文名稱(chēng) | FMO5 Rabbit pAb |
| 中文名稱(chēng) | 二甲基苯胺單加氧酶5抗體 |
| 別 名 | Dimethylaniline monooxygenase [N oxide forming] 5; Dimethylaniline monooxygenase [N-oxide-forming] 5; Dimethylaniline oxidase 5; FMO 5; FMO5; FMO5_HUMAN; Hepatic flavin containing monooxygenase 5; Hepatic flavin-containing monooxygenase 5. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FMO5: 251-350/533 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Flavin containing monooxygenase family consists of five gene products, FMO1-5, that are major enzymatic oxidants involved in the metabolism of various therapeutics. Localizing to microsomal and endoplasmic reticulum membranes, FMO5 (flavin containing monooxygenase 5), also known as dimethylaniline monooxygenase [N-oxide-forming] 5, hepatic flavin-containing monooxygenase 5 or dimethylaniline oxidase 5, is a 533 amino acid protein belonging to the FMO family. Expressed in adult and fetal liver, FMO5 is unlike other FMO family members because it does not function as a drug-metabolizing enzyme. FMO5 binds FAD as a cofactor and is encoded by a gene located on human chromosome 1q21.1. Function: In contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme. Subcellular Location: Microsome membrane. Endoplasmic reticulum membrane. Tissue Specificity: Expressed in fetal and adult liver. Similarity: Belongs to the FMO family. SWISS: P49326 Gene ID: 2330 Database links: Entrez Gene: 2330 Human Entrez Gene: 14263 Mouse Omim: 603957 Human SwissProt: P49326 Human SwissProt: P97872 Mouse Unigene: 642706 Human Unigene: 385180 Mouse Unigene: 7038 Rat |
| 產(chǎn)品圖片 |
Protein: liver(mouse)lyates at 40ug;
Primary: Anti-FMO5 (bs-13187R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size : 60 kD
Observed band size :60 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FMO5 Polyclonal Antibody, Unconjugated(bs-13187R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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