| 產(chǎn)品編號(hào) | bs-16640R |
| 英文名稱 | INDOL1 Rabbit pAb |
| 中文名稱 | 吲哚1抗體 |
| 別 名 | 3-dioxygenase 2; 3-dioxygenase-like protein 1; EC 1.13.11.; I23O2_HUMAN; IDO 2; IDO-2; Ido2; INDOL 1; Indoleamine 2; Indoleamine 2, 3 dioxygenase 2; Indoleamine 2, 3 dioxygenase like 1 protein; Indoleamine 2, 3 dioxygenase like protein 1; Indoleamine pyrrole 2, 3 dioxygenase like 1; Indoleamine pyrrole 2, 3 dioxygenase like protein 1; Indoleamine-pyrrole 2. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human INDOL1: 251-350/407 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]).[supplied by OMIM, Feb 2011] Function: Catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. Tissue Specificity: Detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. Similarity: Belongs to the indoleamine 2,3-dioxygenase family. SWISS: Q6ZQW0 Gene ID: 169355 Database links: Entrez Gene: 169355 Human Omim: 612129 Human SwissProt: Q6ZQW0 Human Unigene: 676257 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Mouse Lung tissue lysates
Lane 4: Mouse Spleen tissue lysates
Lane 5: Rat Liver tissue lysates
Lane 6: Rat Spleen tissue lysates
Primary: Anti-INDOL1 (bs-16640R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 60 kD
Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-INDOL1 Polyclonal Antibody, Unconjugated(bs-16640R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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