| 產品編號 | bs-18553R |
| 英文名稱 | ZCCHC4 Rabbit pAb |
| 中文名稱 | ZCCHC4蛋白抗體 |
| 別 名 | 4930449I23Rik; HSPC052; MGC21108; OTTMUSP00000027189; zcchc4; ZCHC4_HUMAN; Zinc finger CCHC domain containing protein 4; Zinc finger CCHC domain-containing protein 4; Zinc finger, CCHC domain containing 4. |
| 研究領域 | 免疫學 轉錄調節(jié)因子 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Sheep,Cow,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 59 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZCCHC4: 301-400/513 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
ZCCHC4 is a 513 amino acid protein that contains one CCHC-type zinc finger and one DHHC-type zinc finger and is thought to function as a methyltransferase. The gene encoding ZCCHC4 maps to human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. Function: May be a methyltransferase. Similarity: Contains 1 CCHC-type zinc finger. Contains 1 DHHC-type zinc finger. SWISS: Q9H5U6 Gene ID: 29063 Database links: Entrez Gene: 29063 Human Omim: 611792 Human SwissProt: Q9H5U6 Human Unigene: 278945 Human |
| 產品圖片 |
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-ZCCHC4 (bs-18553R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZCCHC4) Polyclonal Antibody, Unconjugated (bs-18553R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
