| 產(chǎn)品編號(hào) | bs-12730R |
| 英文名稱 | PLK2 Rabbit pAb |
| 中文名稱 | 絲氨酸/蘇氨酸蛋白激酶Plk2抗體 |
| 別 名 | hPlk2; hSNK; PLK-2; Plk2; PLK2_HUMAN; Polo like kinase 2; Polo-like kinase 2; Serine/threonine protein kinase PLK2; Serine/threonine protein kinase SNK; Serine/threonine-protein kinase PLK2; Serine/threonine-protein kinase SNK; Serum Induced Kinase; Serum |
| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Zebrafish (predicted: Rat,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 78 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PLK2: 301-400/685 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Function: May play a role in the division of at least some cell types, such as fibroblasts, and could function in embryogenesis, wound healing or neoplasia. Post-translational modifications: Catalytic activity is enhanced by phosphorylation of Thr-239. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. Contains 2 POLO box domains. Contains 1 protein kinase domain. SWISS: Q9NYY3 Gene ID: 10769 Database links: Entrez Gene: 10769 Human Entrez Gene: 20620 Mouse Omim: 607023 Human SwissProt: Q9NYY3 Human SwissProt: P53351 Mouse Unigene: 398157 Human Unigene: 658132 Human Unigene: 380 Mouse Unigene: 12100 Rat |
| 產(chǎn)品圖片 |
Sample: MCF-7(Human) Cell Lysate at 40 ug
Primary: Anti-PLK2 (bs-12730R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 78 kD
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLK2) Polyclonal Antibody, Unconjugated (bs-12730R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLK2) Polyclonal Antibody, Unconjugated (bs-12730R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLK2) Polyclonal Antibody, Unconjugated (bs-12730R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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