產(chǎn)品編號(hào) | bs-20647R |
英文名稱 | RANKL/CD254 Rabbit pAb |
中文名稱 | 骨保護(hù)蛋白配體/破骨細(xì)胞分化因子抗體 |
別 名 | OPGL; CD254; hRANKL2; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; RANKL; Receptor activator of nuclear factor kappa B ligand; sOdf; SOFA; TNF related activation induced cytokine; TNFSF 11; TNFSF11; TRANCE; Tumor necrosis factor ligand superfamily member 11; Osteoprotegerin Ligand; TNF11_HUMAN. |
研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 發(fā)育生物學(xué) |
抗體來(lái)源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Dog,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 35 kDa |
檢測(cè)分子量 | |
細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human RANKL/CD254: 131-230/317 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]. Function: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Subcellular Location: Cytoplasm; Secreted and Cell membrane. Tissue Specificity: Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Post-translational modifications: The soluble form of isoform 1 derives from the membrane form by proteolytic processing. The cleavage may be catalyzed by ADAM17. DISEASE: Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. Similarity: Belongs to the tumor necrosis factor family. SWISS: O14788 Gene ID: 8600 Database links: Entrez Gene: 8600 Human Entrez Gene: 21943 Mouse Omim: 602642 Human SwissProt: O14788 Human SwissProt: O35235 Mouse Unigene: 333791 Human Unigene: 249221 Mouse OPGL骨保護(hù)蛋白配體又稱骨保護(hù)素配體(破骨細(xì)胞發(fā)育刺激因子)。屬腫瘤壞死因子TNF-a家族。 OPGL促進(jìn)破骨細(xì)胞的分化和活性,而OPG抑制這些過(guò)程。骨髓瘤細(xì)胞影響骨髓中這兩種蛋白的生理平衡,是發(fā)生溶骨性病變的根本所在。 |
產(chǎn)品圖片 |
Sample:
U937(Human) Cell Lysate at 40 ug
HUVEC(Human) Cell Lysate at 40 ug
Primary: Anti-RANKL/CD254 (bs-20647R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
Sample:
Lymph node(Mouse) Lysate at 40 ug
Lymph node(Rat) Lysate at 40 ug
Primary: Anti-RANKL’CD254 (bs-20647R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37’50 kD
Observed band size: 50 kD
Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-RANKL/CD254 antibody (bs-20647R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
|
| 中文字幕熟女人妻av一区二区三区 | 性猛交AAAA片免费观看直播 | 国产无码在线观看黄色 | 久久成人99九九电影 | 国产永久精品www嫩草 | 四川寡妇高潮AAA片毛片 | AAA级黄色视频网站 欧美一级婬片A片无码 | 无码A片全身按摩AⅤ | 黄色成人网站在线观看 | 一级a婬片试看30分钟 | 成人短视频在线观看免费 | 国精产品一区二区三区在线观看 | 波多野结衣熟女人妻大脚脚责 | 玩东北老熟女棚户区HD | 欧美搡大BBBB流水 | 日韩精品在线播放 | 波多野结衣av一区二区全免费观看 | 四川妇女一级A片免费看 | 午夜拍拍拍拍拍拍拍拍拍拍拍 | 亚洲AV无码A片在线观看蜜桃 | 欧美交换配乱婬粗大嫩模 | 极品熟女人妻20p白浆出来了 | 岳伦做爰全A片免费 | 羞羞视频最新地址发布页 | 影音先锋无码av在线 | 国产一级a毛一级a看… | 人人干天天搞夜夜爽 | 四虎在线免费观看 | 国产suv精品一区二区6 | 午夜精品久久久久久久免费APP | 人妻无码啪啪AAAAA | 女人自慰免费观看 | 超碰在线国产swag | 国产性猛交 XX 乱照片 | 狠狠躁18三区二区一区 | 中文字幕精品视频观看视频 | 希志爱野亚洲AⅤ在线观看 亚洲一级婬片A片XXX毛 | 四川少BBB搡BBB爽爽爽 | 搡老女人老妇人一级A片 | 精品秘 一区二三区免费雷安胖子 |