產(chǎn)品編號	bsm-33040M
英文名稱	Mouse Anti-lamin B1 antibody
中文名稱	核纖層蛋白B（核內(nèi)參）單克隆抗體
別名	LMNB1_HUMAN; Lamin-B1; LMNB1; LMN2; LMNB; LMN; ADLD; MCPH26;
	Specific References (1) \| bsm-33040M has been referenced in 1 publications. [IF=5.195] Yanqiu Sun. et al. Xinfeng capsule inhibits inflammation and oxidative stress in rheumatoid arthritis by up-regulating LINC00638 and activating Nrf2/HO-1 pathway. J ETHNOPHARMACOL. 2023 Jan;301:115839 WB ; Human. PubMed:36272490
產(chǎn)品類型	內(nèi)參抗體
研究領(lǐng)域	細胞生物染色質(zhì)和核信號信號轉(zhuǎn)導細胞凋亡細胞類型標志物
抗體來源	Mouse
克隆類型	Monoclonal
克隆號	9C11
交叉反應	Human,Rat
產(chǎn)品應用	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	64 kDa
檢測分子量
細胞定位	細胞核
性狀	Liquid
濃度	1mg/ml
免疫原	Recombinant human Lamin B Protein: 314-386/586
亞型	IgG1
純化方法	affinity purified by Protein G
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]. Function: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin Subunit: Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Subcellular Location: Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side Post-translational modifications: B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. DISEASE: Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis. Similarity: Belongs to the intermediate filament family. SWISS: P20700 Gene ID: 4001 Database links: Entrez Gene: 396223 Chicken Entrez Gene: 4001 Human Entrez Gene: 16906 Mouse Entrez Gene: 116685 Rat Omim: 150340 Human SwissProt: P14731 Chicken SwissProt: P20700 Human SwissProt: P14733 Mouse SwissProt: P70615 Rat Unigene: 89497 Human Unigene: 4105 Mouse Unigene: 11362 Rat
產(chǎn)品圖片	Sample: Lane 1: Human Molt-4 cell lysates Lane 2: Human Hela cell lysates Lane 3: Human U-2os cell ysates Primary: Anti-Lamin B (bsm-33040M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa Sample: Lane 1: Rat Pancreas tissue lysates Lane 2: Rat Testis tissue lysates Lane 3: Rat Thymus tissue lysates Lane 4: Human Jurkat cell lysates Lane 5: Human Molt-4 cell lysates Lane 6: Human Hela cell lysates Lane 7: Human U-2os cell ysates Lane 8: Human THP-1 cell lysates Primary: Anti-Lamin B (bsm-33040M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (bsm-33040M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

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	免疫組化常用試劑
	免疫印跡常用試劑
	各種標記二抗

	1、抗體溶解方法
	2、抗體修復方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關(guān)于肽鏈的設(shè)計
	9、多肽的溶解與保存
	10、酶標抗體效價測定程序