| 產(chǎn)品編號 | bs-48024P |
| 英文名稱 | Recombinant human G-CSF protein (Active, HEK293) |
| 中文名稱 | 重組人G-CSF (活性的, Tag free) |
| 別 名 | rhG-CSF; GCSF; Colony stimulating factor 3 (granulocyte); CSF 3; CSF beta; CSF3; Csfg; Filgrastim; GCSA; Granulocyte colony stimulating factor; Lenograstim; Macrophage granulocyte inducer 2; MGC45931; MGI 2; Pluripoietin; Granulocyte Colony Stimulating Factors 3; CSF3_HUMAN. ;Recombinant human G-CSF protein (Active, HEK293) 人G-CSF重組蛋白; 重組人G-CSF; 人重組G-CSF; 重組人G-CSF; 粒細胞集落刺激因子; 集落刺激因子; 粒細胞生長因子; 重組人粒細胞集落刺激因子; |
| 理論分子量 | kDa |
| 檢測分子量 | 21-25 |
| 性 狀 | Lyophilized |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | Human |
| 序 列 | 31-204/207 |
| 純 度 | >95% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內(nèi)毒素 | <0.5 EU/μg as determined by LAL. |
| 表達系統(tǒng) | HEK293 cell |
| 活性 | Yes |
| 標簽 | Tag free |
| 緩 沖 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存條件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Granulocyte colony stimulating factor (G-CSF) is a hematopoietic growth factor belonging to the CSF family. G-CSF is mainly expressed in monocyte/macrophage lineage. It is also expressed in cells of mesodermal origin, including vascular endothelial cells, fibroblasts, and mesothelial cells. G-CSF acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This recombinant human G-CSF is produced by human cells. (Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line). Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| 產(chǎn)品圖片 |
The purity of the protein is greater than 95% as determined by reduced and non reduced SDS-PAGE
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
