| 產(chǎn)品編號 | bsm-60006R |
| 英文名稱 | Glypican 3 Recombinant Rabbit mAb |
| 中文名稱 | 磷脂?；嫉鞍拙厶?3重組兔單抗 |
| 別 名 | GPC3; glypican3; glypican-3; Intestinal protein OCI-5; GTR2-2; MXR7.Intestinal protein OCI-5; GPC3_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1:20-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 細(xì)胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Glypican 3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009] Function: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Subcellular Location: Cell membrane and Secreted, extracellular space. Tissue Specificity: Highly expressed in lung, liver and kidney. DISEASE: Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Similarity: Belongs to the glypican family. SWISS: P51654 Gene ID: 2719 Database links: Entrez Gene: 2719 Human Entrez Gene: 14734 Mouse Omim: 300037 Human SwissProt: P51654 Human SwissProt: Q8CFZ4 Mouse Unigene: 644108 Human Unigene: 22515 Mouse Unigene: 9717 Rat GPC-3磷脂酰基醇蛋白聚糖-3是一種硫酸類肝素蛋白多糖，GPC-3在不同的組織中具有不同的生物學(xué)功能，甚至起著完全不同的作用，可能與腫瘤組織類型、發(fā)病機(jī)制等密切相關(guān)．GPC3在絕大多數(shù)HCC(肝細(xì)胞癌)患者有表達(dá)。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Glypican 3 monoclonal antibody, unconjugated (bsm-60006R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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