| 產(chǎn)品編號(hào) | bsm-60223R |
| 英文名稱 | BCL6 Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | C4E12 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:5000,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 轉(zhuǎn)錄抑制因子主要用于生發(fā)中心（GC）的形成和抗體親和力的成熟，其作用機(jī)制與譜系和生物學(xué)功能有關(guān)。在基因毒性應(yīng)激中，p53/TP53依賴性和非依賴性控制GC B細(xì)胞的細(xì)胞周期阻滯。此外，還可以通過改變選擇性NOTCH靶點(diǎn)（如HES5）的NOTCH依賴性轉(zhuǎn)錄復(fù)合物的組成來控制神經(jīng)發(fā)生，包括去乙酰化酶SIRT1的募集，并導(dǎo)致表觀遺傳沉默，神經(jīng)元分化。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Primary: Anti-BCL6 (bsm-60223R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: kDa
Observed band size: 85 kDa
4% Paraformaldehyde-fixed Daudi (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (BCL6) monoclonal Antibody, unconjugated (bsm-60223R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Daudi (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-BCL6 antibody (bsm-60223R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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