| 產(chǎn)品編號 | bsm-60580R |
| 英文名稱 | KDM4B Recombinant Rabbit mAb |
| 中文名稱 | 賴氨酸特異性脫甲基酶4B重組兔單抗 |
| 別 名 | KDM4B_HUMAN; Lysine-specific demethylase 4B; JHDM3B; JMJD2B; KIAA0876; EC:1.14.11.66; JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B; [histone H3]-trimethyl-L-lysine(9) demethylase 4B; lysine demethylase 4B; |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | G8C10 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:1000,Flow-Cyt=1:50-1:100,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 122 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Enables histone H3-methyl-lysine-36 demethylase activity and histone H3-methyl-lysine-9 demethylase activity. Involved in histone H3-K36 demethylation and histone H3-K9 demethylation. Located in cytosol and nucleoplasm. Implicated in autosomal dominant non-syndromic intellectual disability; breast cancer; colorectal cancer; malignant peripheral nerve sheath tumor; and stomach cancer. Biomarker of several diseases, including alopecia areata; lung cancer; medulloblastoma; prostate cancer; and stomach cancer. [provided by Alliance of Genome Resources, Apr 2022] Subcellular Location: Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin. Tissue Specificity: Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. Database links: Entrez Gene: 23030 Human SwissProt: O94953 Human 賴氨酸脫甲基酶4B（KDM4B）是一種組蛋白去甲基化酶，它能催化H3K9me3的去甲基化反應(yīng)，松弛染色質(zhì)結(jié)構(gòu)和允許轉(zhuǎn)錄因子進(jìn)入，即通過表觀遺傳方式調(diào)節(jié)基因表達(dá)。組蛋白賴氨酸殘基的甲基化狀態(tài)對于基因組激活和非激活區(qū)域的形成是一個主要決定因素，并且在發(fā)育期間對于基因組的正確編碼發(fā)揮關(guān)鍵作用。研究發(fā)現(xiàn)，KDM4B可能在 ERα 結(jié)合位點刪除抑制性組蛋白標(biāo)記，為重塑染色質(zhì)的酶和轉(zhuǎn)錄因子產(chǎn)生錨定位點，進(jìn)而促進(jìn) ERα 介導(dǎo)的轉(zhuǎn)錄。此外，過表達(dá)KDM4B通過對H3K9me3的去甲基化會導(dǎo)致LINE-1拷貝數(shù)、轉(zhuǎn)座活性和DNA損傷程度增加，而KDM4B抑制劑的使用抑制了LINE-1介導(dǎo)的DNA損傷。KDM4B在在乳腺癌、結(jié)腸癌、卵巢癌、肺癌和前列腺癌中均有高表達(dá)，其致病機(jī)制為腫瘤的預(yù)防和靶向治療提供線索。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: T47D, 2: LNCap, 3: K562
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 122 kDa
Observed MW: 150 kDa
Cell line: K562
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60580R
Cell line: K562
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60580R
|
