| 產(chǎn)品編號 | bsm-60599R |
| 英文名稱 | GOT1 Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | H4D9 |
| 交叉反應 | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應用 | WB=1:500-1:1000,IHC-P=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 從L-天冬氨酸或L-半胱氨酸生物合成L-谷氨酸。谷氨酸水平的重要調節(jié)者，谷氨酸是脊椎動物中樞神經(jīng)系統(tǒng)的主要興奮性神經(jīng)遞質。在腦神經(jīng)保護中起到谷氨酸清除劑的作用。天門冬氨酸氨基轉移酶活性參與發(fā)育過程中肝臟葡萄糖的合成和脂肪細胞的新生。以L-半胱氨酸為底物，調節(jié)巰基丙酮酸的水平，巰基丙酮酸是硫化氫的重要來源。巰基丙酮酸通過3-巰基丙酮酸硫轉移酶（3MST）的作用轉化為H2S。硫化氫是腦內(nèi)重要的突觸調節(jié)劑和神經(jīng)保護劑。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: Mouse heart, 3: Rat heart
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 46 kDa
Observed MW: 46 kDa
Tissue: Rat kidney
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60599R
Tissue: Human cerebrum
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60599R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
