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GART Mouse mAb (bsm-60639M)  
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產(chǎn)品編號(hào) bsm-60639M
英文名稱 GART Mouse mAb
中文名稱 甘氨酰胺核苷酸合成酶單克隆抗體
別    名 5'-phosphoribosylglycinamide transformylase; AIR synthase; AIRS; GAR transformylase; GARS; GARTF; Glycinamide ribonucleotide synthetase; MGC47764; PAIS; PGFT; phosphoribosyl-aminoimidazole synthetase; phosphoribosylglycinamide formyltransferase; phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase; phosphoribosylglycinamide formyltransferase, EC 2.1.2.29; phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase; phosphoribosylglycinamide synthetase; PRGS; PUR2_HUMAN; Trifunctional purine biosynthetic protein adenosine 3.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  信號(hào)轉(zhuǎn)導(dǎo)  新陳代謝  表觀遺傳學(xué)  
抗體來(lái)源 Mouse
克隆類型 Monoclonal
克 隆 號(hào) 1D14
交叉反應(yīng) Human (predicted: Rat)
產(chǎn)品應(yīng)用 WB=1:500-1000,ICC/IF=1:50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 107 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GART 
亞    型 IgG1,Kappa
純化方法 affinity purified by Protein A
緩 沖 液 PBS, Glycerol, BSA.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Purines are critical for energy metabolism, cell signaling and cell reproduction and also function as precursors for coenzymes, energy transfer molecules, regulatory factors and proteins involved in RNA and DNA synthesis. GART (GAR transformylase), also referred to as AIRS, GARS, PAIS, PGFT, PRGS or GARTF, is 1,010 amino acids in length and is a key folate-dependent trifunctional enzyme with phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and AICAR (phosphoribosylaminoimidazole synthetase) activity required for de novo purine biosynthesis. Cancer cells require considerable amounts of purines to sustain their accelerated growth and GART is, therefore, a target for cancer chemotherapy. GART is highly conserved in vertebrates. Two isoforms of GART are expressed due to alternative splicing events.

SWISS:
P22102

Gene ID:
2618

Database links:

Entrez Gene: 2618 Human

Entrez Gene: 14450 Mouse

Entrez Gene: 288259 Rat

Omim: 138440 Human

SwissProt: P22102 Human

SwissProt: Q64737 Mouse

Unigene: 473648 Human

Unigene: 4505 Mouse



產(chǎn)品圖片
Sample: Lane 1: Human HeLa cell lysates Lane 2: Human A431 cell lysates Lane 3: Human HepG2 cell lysates Primary: Anti-GART (bsm-60639M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution Predicted band size: 107 kDa Observed band size: 55,130 kDa
Cell line: HepG2 Fixation: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for bsm-60639M
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