| 產(chǎn)品編號 | bsm-60664R |
| 英文名稱 | HSD17B1 Recombinant Rabbit mAb |
| 中文名稱 | 羥類固醇脫氫酶17β-HSD重組兔單抗 |
| 別 名 | 11beta-HSD1; 17beta-HSD1; 17 beta HSD 1; 17 beta hydroxysteroid dehydrogenase type 1; 17β-hydroxysteroid dehydrogenasetype 1 EDH17B2; EDHB17; Estradiol 17 beta dehydrogenase 1; HSD17; Hydroxysteroid(17 beta) dehydrogenase 1; Placental 17 beta hydroxysteroid dehydrogenase; DHB1_HUMAN. |
| 研究領(lǐng)域 | 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | R6D3 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-5000,IHC-P=1:100-500,IHC-F=1:100-400,IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HSD17B1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016] Subcellular Location: Cytoplasm. SWISS: P14061 Gene ID: 3292 Database links: Entrez Gene: 3292 Human Omim: 109684 Human SwissProt: P14061 Human Unigene: 654385 Human Unigene: 655222 Human |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:20000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: Human heart (Negative control), 2:
Human placenta
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 35 kDa
Observed MW: 35 kDa
Tissue: Human prostatic hyperplasia (Negative
tissue)
Section type: Formalin-fixed &
Paraffin-embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: No staining on bsm-60664R
Tissue: Human placenta
Section type: Formalin-fixed &
Paraffin-embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
bsm-60664R
|
