產(chǎn)品編號 | bsm-60682R |
英文名稱 | ORAI3 Recombinant Rabbit mAb |
中文名稱 | 嗅覺受體AI3重組兔單抗 |
別 名 | ORAI calcium release activated calcium modulator 3; ORAI3_HUMAN; Protein orai 3; Protein orai-3; TMEM 142C; TMEM142C; Transmembrane protein 142C. |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | R5C12 |
交叉反應 | Human |
產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 31 kDa |
檢測分子量 | |
細胞定位 | 細胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ORAI3 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Predicted to enable store-operated calcium channel activity. Predicted to be involved in store-operated calcium entry. Predicted to be located in plasma membrane. Predicted to be integral component of membrane. Predicted to be active in membrane. [provided by Alliance of Genome Resources, Apr 2022] Subcellular Location: Membrane. SWISS: Q9BRQ5 Gene ID: 93129 Database links:
Entrez Gene: 93129 Human Entrez Gene: 269999 Mouse SwissProt: Q9BRQ5 Human SwissProt: Q6P8G8 Mouse
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產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: A431, 2: A549, 3: Raji, 4: NIH/3T3
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 32 kDa
Observed MW: 32 kDa
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |