| 產(chǎn)品編號(hào) | bsm-55529R |
| 英文名稱 | p38 Recombinant Rabbit mAb |
| 中文名稱 | p38 MAPK重組兔單抗 |
| 別 名 | MAPK14, CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA, mitogen-activated protein kinase 14 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | SB94 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1: 500-2000,IHC-P=1: 50-200,IHC-F=1: 50-200,IF=1: 50-200,Flow-Cyt=1ug/Test,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測(cè)分子量 | 40 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human p38 |
| 亞 型 | IgG |
| 純化方法 | Affinity Purification |
| 緩 沖 液 | PBS with 100 μg/ml BSA,0.15% ProClin300 and 50% glycerol. |
| 保存條件 | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. SWISS: Q16539 Gene ID: 1432 p38 MAP 激酶(MAPK)也稱為 RK 或 CSBP，參與控制細(xì)胞因子和應(yīng)激引起的細(xì)胞反應(yīng)的信號(hào)轉(zhuǎn)導(dǎo)級(jí)聯(lián)。現(xiàn)已發(fā)現(xiàn)p38 的四種同工型：p38α、β、γ（也稱 Erk6 或 SAPK3）和 δ（也稱 SAPK4）。與 SAPK/JNK 通路相似，p38 MAPK 被多種細(xì)胞應(yīng)激激活，包括滲透壓休克、炎性細(xì)胞因子、脂多糖(LPS)、紫外線和生長(zhǎng)因子。MKK3、MKK6 和 SEK 通過(guò)磷酸化 Thr180 和 Tyr182 位點(diǎn)激活 p38 MAPK。激活的 p38 MAPK 已證實(shí)可磷酸化和激活 M |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with p38 monoclonal antibody, unconjugated (bsm-55529R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p38 Monoclonal Antibody, Unconjugated(bsm-55529R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (p38) monoclonal Antibody, unconjugated (bsm-55529R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-p38 antibody (bsm-55529R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
|
