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Rabbit Anti-Transferrin receptor/CD71/BF647 Conjugated antibody (bs-1782R-BF647)
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說(shuō) 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-1782R-BF647
英文名稱 Rabbit Anti-Transferrin receptor/CD71/BF647 Conjugated antibody
中文名稱 BF647標(biāo)記的轉(zhuǎn)鐵蛋白受體抗體
別    名 CD 71; CD71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin Receptor Protein 1; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; Transferrin receptor protein 1, serum form; TRFR.  
規(guī)格價(jià)格 100ul/2980元 購(gòu)買        大包裝/詢價(jià)
說(shuō) 明 書 100ul  
研究領(lǐng)域 腫瘤  心血管  細(xì)胞生物  免疫學(xué)  神經(jīng)生物學(xué)  干細(xì)胞  糖尿病  細(xì)胞表面分子  細(xì)胞類型標(biāo)志物  淋巴細(xì)胞  t-淋巴細(xì)胞  b-淋巴細(xì)胞  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,  (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, )
產(chǎn)品應(yīng)用 Flow-Cyt=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 74/85kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD71
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.

Function:
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferring for an overlapping C-terminal binding site.

Subunit:
Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with Machupo arenavirus GPC.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Transferrin receptor protein 1, serum form: Secreted

Post-translational modifications:
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

Similarity:
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.

Database links:

Entrez Gene: 7037 Human

Entrez Gene: 22042 Mouse

Entrez Gene: 64678 Rat

Omim: 190010 Human

SwissProt: P02786 Human

SwissProt: Q62351 Mouse

SwissProt: Q99376 Rat

Unigene: 529618 Human

Unigene: 28683 Mouse

Unigene: 98672 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

轉(zhuǎn)鐵蛋白及轉(zhuǎn)鐵蛋白受體(TfnrR)是機(jī)體鐵代謝中的重要因子,鐵是細(xì)胞生長(zhǎng)增殖、代謝的必需元素,轉(zhuǎn)鐵蛋白受體(TfR)介導(dǎo)的胞吞作用是細(xì)胞攝取鐵的主要途徑。腫瘤細(xì)胞因增殖生長(zhǎng)旺盛,對(duì)鐵的需求高,所以腫瘤細(xì)胞有比正常細(xì)胞更多的轉(zhuǎn)鐵蛋白受體的表達(dá)。
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