| 產(chǎn)品編號 | bs-7523R-Gold |
| 英文名稱 | Rabbit Anti-phospho-Caveolin-2 (Tyr19)/Gold Conjugated antibody |
| 中文名稱 | 膠體金標(biāo)記的磷酸化細(xì)胞質(zhì)膜微囊蛋白-2抗體 |
| 別 名 | Caveolin 2 (phospho Y19); p-Caveolin 2(phospho Y19); CAV; CAV2; CAV2_HUMAN; Caveolae protein 20 kD; Caveolin 2; Caveolin 2 isoform a and b; Caveolin 2 isoform c; Caveolin-2; MGC12294; CAV2_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul（10nm 15nm 35nm） |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 心血管 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, Rat, |
| 產(chǎn)品應(yīng)用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 18kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from mouse Caveolin2 around the phosphorylation site of Tyr19. |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產(chǎn)品介紹 |
background: Cellular localization: Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments. Function: May act as a scaffolding protein within caveolarmembranes. Interacts directly with G-protein alpha subunits and canfunctionally regulate their activity. Acts as an accessory proteinin conjunction with CAV1 in targeting to lipid rafts and drivingcaveolae formation. The Ser-36 phosphorylated form has a role inmodulating mitosis in endothelial cells. Positive regulator ofcellular mitogenesis of the MAPK signaling pathway. Required forthe insulin-stimulated nuclear translocation and activation ofMAPK1 and STAT3, and the subsequent regulation of cell cycleprogression (By similarity). Subunit: Monomer or homodimer. Interacts with CAV1; theinteraction forms a stable heterooligomeric complex that isrequired for targeting to lipid rafts and for caveolae formation.Tyrosine phosphorylated forms do not form heterooligomers with theTyr-19-phosphorylated form existing as a monomer or dimer, and theTyr-27-form as a monomer only. Interacts (tyrosine phosphorylatedform) with the SH2 domain-containing proteins, RASA1, NCK1 and SRC.Interacts (tyrosine phosphorylated form) with INSR, the interaction(Tyr-27-phosphorylated form) is increased on insulin stimulation.Interacts (Tyr-19 phosphorylated form) with MAPK1 (phosphorylatedform); the interaction, promoted by insulin, leads to nuclearlocation and MAPK1 activation. Interacts with STAT3; theinteraction is increased on insulin-induced tyrosinephosphorylation leading to STAT activation (By similarity). Subcellular Location: Nucleus. Cytoplasm. Golgi apparatusmembrane; Peripheral membrane protein. Cell membrane; Peripheralmembrane protein. Membrane, caveola; Peripheral membrane protein.Note=Potential hairpin-like structure in the membrane. Membraneprotein of caveolae. Tyr-19-phosphorylated form is enriched atsites of cell-cell contact and is translocated to the nucleus incomplex with MAPK1 in response to insulin (By similarity).Tyr-27-phosphorylated form is located both in the cytoplasm andplasma membrane. CAV1-mediated Ser-23-phosphorylated form locatesto the plasma membrane. Ser-36-phosphorylated form resides inintracellular compartments. Tissue Specificity: Expressed in endothelial cells, smooth musclecells, skeletal myoblasts and fibroblasts. Post-translational modifications: Phosphorylated on serine and tyrosine residues. CAV1 promotesphosphorylation on Ser-23 which then targets the complex to theplasma membrane, lipid rafts and caveolae. Phosphorylation onSer-36 appears to modulate mitosis in endothelial cells (Bysimilarity). Phosphorylation on both Tyr-19 and Tyr-27 is requiredfor insulin-induced 'Ser-727' phosphorylation of STAT3 and itsactivation. Phosphorylation on Tyr-19 is required forinsulin-induced phosphorylation of MAPK1 and DNA binding of STAT3.Tyrosine phosphorylation is induced by both EGF and insulin (Bysimilarity). Similarity: Belongs to the caveolin family. Database links: Entrez Gene: 858 Human Entrez Gene: 12390 Mouse Omim: 601048 Human SwissProt: P51636 Human SwissProt: Q9WVC3 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Caveolin是細(xì)胞生長相關(guān)信號途徑及腫瘤發(fā)生發(fā)展過程中重要的抑制因子，Caveolae是細(xì)胞膜內(nèi)的特殊膜結(jié)構(gòu)，參與包括細(xì)胞的分子運(yùn)輸、細(xì)胞粘附和信號轉(zhuǎn)導(dǎo)在內(nèi)的多種細(xì)胞活動。Caveolin-1是Caveolae中重要的結(jié)構(gòu)蛋白，抑制細(xì)胞生長，與多種人類腫瘤發(fā)生發(fā)展相關(guān)的信號分子相互作用。Caveolin在信號轉(zhuǎn)導(dǎo)的整合中起支架蛋白的作用。Caveolin構(gòu)成了一個蛋白家族，他們是細(xì)胞質(zhì)膜中發(fā)夾樣結(jié)構(gòu)域的主要結(jié)構(gòu)成分。Caveolin在信號轉(zhuǎn)導(dǎo)的整合中起支架蛋白的作用。至今已經(jīng)鑒定了3種Caveolin （Caveolin-1、2 和3），它們具有不同的組織分布。 |
