產(chǎn)品編號(hào) | bs-2775R-Bio |
英文名稱(chēng) | Rabbit Anti-LIMK1/Biotin Conjugated antibody |
中文名稱(chēng) | 生物素標(biāo)記的單絲氨酸蛋白激酶1抗體 |
別 名 | EC 2.7.11.1; LIM domain kinase 1; LIM motif-containing protein kinase; LIMK; LIMK-1; LIMK2; LIMK-2; LIM domain kinase 2; LIMK1_HUMAN. |
規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買(mǎi) 大包裝/詢(xún)價(jià) |
說(shuō) 明 書(shū) | 100ul |
研究領(lǐng)域 | 腫瘤 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 表觀遺傳學(xué) |
抗體來(lái)源 | Rabbit |
克隆類(lèi)型 | Polyclonal |
交叉反應(yīng) | Mouse, Rat, (predicted: Human, Cow, Rabbit, ) |
產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 71kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human LIMK1 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. LIMK1 may be involved in brain development; it is highly expressed in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. Function: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Subunit: Interacts (via LIM domain) with the cytoplasmic domain of NRG1. Interacts with NISCH. Interacts with RLIM and RNF6. Self-associates to form homodimers. Interacts with HSP90AA1; this interaction promotes LIMK1 dimerization and subsequent transphosphorylation. Interacts with CDN1C. Interacts with SSH1. Interacts with ROCK1. Subcellular Location: Cytoplasm. Nucleus. Note=Predominantly found in the cytoplasm. Tissue Specificity: Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. Post-translational modifications: Autophosphorylated. Phosphorylated on Thr-508 by ROCK1 and PAK1, resulting in activation. Phosphorylated by PAK4 which increases the ability of LIMK1 to phosphorylate cofilin. Phosphorylated at Ser-323 by MAPKAPK2 during activation of VEGFA-induced signaling, which results in activation of LIMK1 and promotion of actin reorganization, cell migration, and tubule formation of endothelial cells. Dephosphorylated and inactivated by SSH1. Phosphorylated by CDC42BP. Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM. DISEASE: Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 2 LIM zinc-binding domains. Contains 1 PDZ (DHR) domain. Contains 1 protein kinase domain. Database links: Entrez Gene: 3984 Human Entrez Gene: 16885 Mouse Omim: 601329 Human SwissProt: P53667 Human SwissProt: P53668 Mouse Unigene: 647035 Human Unigene: 15409 Mouse Unigene: 11250 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. LIM激酶1(LIMkinase1,LIMK-1)是單絲氨酸蛋白激酶,LIMK-1的活化受多種機(jī)制調(diào)控,成為聯(lián)系細(xì)胞外刺激與細(xì)胞骨架穩(wěn)定性的樞紐,在多種基本的生物學(xué)過(guò)程中起重要作用。LIMK-1包含LIM和PDZ蛋白和蛋白相互作用區(qū)、P/S結(jié)構(gòu)域、激酶結(jié)構(gòu)域,在神經(jīng)元中呈高表達(dá)。它的主要作用是使肌動(dòng)蛋白解聚因子cofilin磷酸化和失活,從而導(dǎo)致肌動(dòng)蛋白細(xì)胞骨架的改變。 |
| 熟女岳胥乱一区二区三区免费看 | 国产精品无码专区 | 一级黄色影片7777 | 国产伦子伦一级A片在线 | 日韩黄色电影在线观看 | 色诱女教师一区二区三区 | 国产成人精品 水 国产成人精品AA毛片 | 久久久免费视频闷站 | 亚洲精品无码久久久字幕网站 | 少妇熟女_第68页 | 又黑又粗又长黄色视频 | 四川一级毛片免费看 | 极品白丝自慰喷水 | 色婷婷一区二区三区久久午夜成人 | 成人四虎成人中文综合 | 蜜桃精品一区二区三区 | 日韩免费一级无码婬片AA片软件 | 99视频精品免费 | 亂倫近親相姦免费中文字幕 | 国产精品妖精久久人妻嫩 | 插美女在酒店免费网站视频 | 又大又长又粗又爽视频 | 亚州精品一区二区三区黄久 | 西西大胆色情一区二区三区 | 欧美一级婬片a毛片无码 | 北条麻妃在线视频 | 婬荡欲女搡BBBB搡BBB | 亚欧精品久久人人妻人人爽 | 一级女婬片A片AAAA片 | 无码人妻丰满熟妇精品区 | 在线观看午夜成人一区二区三区 | 亚洲熟妇AⅤ一区二区三区 国产A三级三级三级看三级 | 精品人妻一区二区三区阅读全文 | 30分钟免费婬片A片 黄色美女视频在线观看 | 日韩丝袜人妻 中文字幕 | 少妇被又大又粗又爽久久 | 又大又黄又爽无码 | 亚洲一区二区三区在线 | av无码在线播放 | 国产农村伊人AV色小U女 |