| 產(chǎn)品編號(hào) | bs-0013R-FITC |
| 英文名稱 | Rabbit Anti-Cytochrome C/FITC Conjugated antibody |
| 中文名稱 | FITC標(biāo)記的細(xì)胞色素C抗體 |
| 別 名 | CytC; CYC; CYCS; Cytochrome c somatic; HCS; CYC_HUMAN; Cytochrome c; MSA06; THC4. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買 大包裝/詢價(jià) |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 心血管 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 脂蛋白 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Chicken, Pig, Cow, Horse, Rabbit, Guinea Pig, ) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 12.8/26kDa |
| 細(xì)胞定位 | 細(xì)胞膜 線粒體 |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cytochrome C |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis. Function: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. Subcellular Location: Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane. Post-translational modifications: Binds 1 heme group per subunit. Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration. DISEASE: Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation. Similarity: Belongs to the cytochrome c family. Database links: Entrez Gene: 54205 Human Entrez Gene: 13063 Mouse Omim: 123970 Human SwissProt: P99999 Human SwissProt: P62897 Mouse Unigene: 437060 Human UniProtKB/Swiss-Prot: P99999.2
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 細(xì)胞色素C（cytC）是一種電子傳遞鏈蛋白為線粒體呼吸鏈必須的成份之一。在哺乳動(dòng)物細(xì)胞中，如此高度保守性蛋白常分布在線立體內(nèi)膜。 新近研究證明細(xì)胞漿中細(xì)胞色素C為激活細(xì)胞調(diào)亡所必需的因子。在調(diào)亡的過程中，細(xì)胞色素C從線立體膜被易位到細(xì)胞漿，由細(xì)胞色素C激活Caspase-3（CPP32）。 細(xì)胞色素C的易位可被過量表達(dá)的Bcl-2阻斷。細(xì)胞色素B與細(xì)胞色素C1和Rieske蛋白相結(jié)合而形成復(fù)合物III（也稱細(xì)胞色素B-C1復(fù)合物）參與細(xì)胞呼吸鏈。該蛋白動(dòng)物種屬間同源性較高；如 ：豬、犬、牛、雞、豚鼠等。 |
| 產(chǎn)品圖片 |
Blank control:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Cytochrome C antibody (bs-0013R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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