產(chǎn)品編號 | bs-0596R-AP |
英文名稱 | Rabbit Anti-Dopamine beta Hydroxylase/AP Conjugated antibody |
中文名稱 | 堿性磷酸酶(AP)標記的多巴胺β羥化酶抗體 |
別 名 | DBH; DBM; Dopamine beta hydroxylase; Dopamine-Beta-Hydroxylase; Dopamine beta monooxygenase; DOPO_RAT; DOPO_HUMAN. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 腫瘤 神經(jīng)生物學 合成與降解 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human, (predicted: Mouse, Rat, ) |
產(chǎn)品應用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 70kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human DBH |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Dopamine-beta-Hydroxylase (DBH), Dopamine can be transformed into Noradrenalin. Conversion of dopamine to noradrenaline. [SUBUNIT] Homotetramer composed of two non-covalently bound disulfide-linked dimers. [SUBCELLULAR LOCATION] Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (Potential). Belongs to the copper type II ascorbate-dependent monooxygenase family. Function: Conversion of dopamine to noradrenaline. Subunit: Homotetramer composed of two non-covalently bound disulfide-linked dimers (By similarity). Subcellular Location: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen (By similarity). Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (By similarity). Tissue Specificity: Chromaffin granules of the adrenal medulla and synaptic vesicles of the sympathetic nervous system. Similarity: Belongs to the copper type II ascorbate-dependent monooxygenase family. Contains 1 DOMON domain. Database links: Entrez Gene: 1621 Human Entrez Gene: 13166 Mouse Omim: 223360 Human Omim: 609312 Human SwissProt: P09172 Human SwissProt: Q64237 Mouse Unigene: 223858 Human Unigene: 591890 Human Unigene: 167781 Mouse Unigene: 87166 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Dopamine-β-Hydroxylase(DBH)—多巴胺β羥化酶??梢詫⒍喟桶忿D(zhuǎn)化成去甲腎上腺素。是腎上腺素能神經(jīng)元的標志。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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